Human FGF-21 Quantikine ELISA Kit | hFGF-21 QKit
掲載日情報：2016/08/18 現在Webページ番号：111082
R&D Systems製のHuman FGF-21を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年08月28日 08時55分現在]
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FGF-21, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年08月28日 08時55分現在]
FGF-21, Human, ELISA Kit, Quantikine (96well)
文献数： 0
- 商品コード：DF2100
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.3-2,000 pg/mL，感度：8.69 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC24，測定時間：4.5 hours，測定因子：FGF-21 別名：FGF21 Genbank No： 26291 |
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| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2021年5月1日号 p.7
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| 製品記事 | Human FGF-21 Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit R&D Systems製品 価格改定（値下げ）のご案内 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 8.69 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human FGF-21
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 204 | 672 | 1,324 | 245 | 742 | 1,411 |
| Standard Deviation | 8 | 19.8 | 46.2 | 26.6 | 47.2 | 73.9 |
| CV% | 3.9 | 2.9 | 3.5 | 10.9 | 6.4 | 5.2 |
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Recovery
The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 105 | 87-119 |
| Heparin Plasma (n=4) | 98 | 83-111 |
| EDTA Plasma (n=4) | 103 | 92-112 |
| Cell Culture Media (n=4) | 98 | 93-108 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- FGF-21
- Aliases
- undefined
- Entrez Gene IDs
- 26291 (Human); 56636 (Mouse)
- Background
- FGF-21
- FGF-21 is a 209 aa polypeptide that presumably has a signal sequence and is secreted. Human FGF-21 demonstrates only 75% aa identity with mouse FGF-21. In mouse, it is expressed preferentially in liver.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history.
An C, Li J, Wang D, Wang Z, Zhu W
PLoS ONE 2013 8:e81190
Species: Human
Sample Type: Serum
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