Human EGF R/ErbB1 Quantikine ELISA Kit | hEGF R QKit
掲載日情報：2016/08/18 現在Webページ番号：111075
R&D Systems製のHuman EGF R/ErbB1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年06月29日 00時00分現在]
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EGF Receptor, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年06月29日 00時00分現在]
EGF Receptor, Human, ELISA Kit, Quantikine (96well)
文献数： 5
- 商品コード：DEGFR0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.313-20 ng/mL，感度：0.036 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Human Milk，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC79，測定時間：4.5 hours，測定因子：EGFR 別名：avian erythroblastic leukemia viral (v-erb-b) oncogene homolog Genbank No： 1956 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Human EGF R/ErbB1 Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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関連製品
[在庫・価格 ：2025年06月29日 00時00分現在]
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Phospho-Kinase Array Kit, Human, Proteome Profiler |
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本製品は製品内容等が変更されました
変更先製品 |
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[在庫・価格 ：2025年06月29日 00時00分現在]
Phospho-Kinase Array Kit, Human, Proteome Profiler
文献数： 212
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | Proteome Profiler Human Phospho-Kinase Array Kit |
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| 関連記事 | R&D Systems社製品#ARY003Bの販売終了および改良版#ARY003Cの販売 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Human Milk (50 µL)
- Sensitivity
- 0.036 ng/mL
- Assay Range
- 0.313 - 20 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human EGF R
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human EGF R immunoassay is a 4.5 hour solid phase ELISA designed to measure EGF R in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human EGF R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural EGF R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human EGF R.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.69 | 5.33 | 11.2 | 1.7 | 5.21 | 10.4 |
| Standard Deviation | 0.072 | 0.199 | 0.616 | 0.161 | 0.522 | 1.03 |
| CV% | 4.3 | 3.7 | 5.5 | 9.4 | 10 | 10 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.65 | 5.08 | 10.6 | 1.63 | 5 | 10 |
| Standard Deviation | 0.094 | 0.17 | 0.507 | 0.144 | 0.416 | 0.816 |
| CV% | 5.7 | 3.3 | 4.8 | 8.8 | 8.3 | 8.2 |
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Recovery
The recovery of EGF R spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 101 | 89-117 |
| Heparin Plasma (n=4) | 101 | 92-111 |
| EDTA Plasma (n=4) | 99 | 91-108 |
| Cell Culture Media (n=4) | 98 | 86-106 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of EGF R were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- EGF R/ErbB1
- Aliases
- ErbB; ErbB1; HER-1
- Entrez Gene IDs
- 1956 (Human); 13649 (Mouse)
- Background
- EGF Receptors
- The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites. The human EGF R cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse. Within the ECD, human EGF R shares 88% aa sequence identity with mouse and rat EGF R. It shares 43% - 44% aa sequence identity with the ECD of human ErbB2, ErbB3, and ErbB4. EGF R binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGF R is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor.
Bentley , Victoria, Garnier , Delphine, Kislinger , Thomas, Lee , Tae Hoon, Magnus , Nathalie, Meehan , Brian, Milsom , Chloe, Montermini , Laura, Rak , Janusz
J Biol Chem 2012 287:43565-72
Species: Human
Sample Type: Cell Culture Supernates
Morphological and biochemical features of obesity are associated with mineralization genes' polymorphisms.
Cohen Z, Korostishevsky M, Malkin I
Int J Obes (Lond) 2010 34:1308-18
Species: Human
Sample Type: Plasma
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