Human EGF Quantikine ELISA Kit | hEGF QKit
掲載日情報：2016/08/18 現在Webページ番号：111074
R&D Systems製のHuman EGFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月28日 16時55分現在]
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EGF, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月28日 16時55分現在]
EGF, Human, ELISA Kit, Quantikine (96well)
文献数： 85
- 商品コード：DEG00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：3.9-250 pg/mL，感度：0.7 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC321，測定時間：3.5 hours or 4.5 hours，測定因子：EGF 別名：beta-urogastrone Genbank No： 1950 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours or 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (10 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Platelet-poor Citrate Plasma (200 µL), Urine (10 µL)
- Sensitivity
- 0.7 pg/mL
- Assay Range
- 3.9 - 250 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Platelet-poor Citrate Plasma, Urine)
- Specificity
- Natural and recombinant human EGF
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 13.5 | 80 | 133 | 14.7 | 77 | 134 |
| Standard Deviation | 0.6 | 2 | 2.7 | 0.9 | 3.3 | 6.1 |
| CV% | 4.4 | 2.5 | 2 | 6.1 | 4.3 | 4.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 15 | 78.9 | 134 | 15 | 80 | 136 |
| Standard Deviation | 0.4 | 2.5 | 4 | 0.9 | 4.5 | 6.8 |
| CV% | 2.7 | 3.2 | 3 | 6 | 5.6 | 5 |
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Recovery
The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=10) | 97 | 80-106 |
| Heparin Plasma (n=10) | 96 | 79-108 |
| Citrate Plasma (n=10) | 104 | 89-115 |
| EDTA Plasma (n=10) | 100 | 80-111 |
| Urine (n=1) | 102 | 97-108 |
| Cell Culture Media (n=5) | 98 | 88-111 |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of EGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- EGF
- Aliases
- HOMG4; URG; Urogastrone
- Entrez Gene IDs
- 1950 (Human); 13645 (Mouse); 25313 (Rat)
- Background
- EGF
- Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands. Its actions were shown to induce early eyelid opening, incisor eruption, hair growth inhibition, and stunt growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.A wide variety of in vitro and in vivo biological effects have been ascribed to EGF and other members of the EGF family. The best documented activity of EGF is its ability to promote proliferation and differentiation of mesenchymal and epithelial cells. In vitro, EGF is a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells in culture. In vivo, EGF induces epithelial development, promotes angiogenesis, and inhibits gastric acid secretion.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma
Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost. 2012 10:1616-23
Species: Human
Sample Type: Serum
Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Bork U, Buchler MW, Falk CS, Herber M, Hinz U, Koch M, Rahbari NN, Schmidt T, Weitz J
BMC Cancer 2011 11:286
Species: Human
Sample Type: Serum
Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia 2011 54:2358-67
Species: Human
Sample Type: Serum
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Amann EM, Feifel E, Gstraunthaler G, Pfaller W, Rauch C, Schennach H, Spotl HP
ALTEX 2011 28:305-16
Species: Human
Sample Type: Cell Lysates
Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study.
Cherney DZ, Diamandis EP, Konvalinka A, Lai V, Lorraine J, Miller JA, Reich H, Scholey JW, Soosaipillai A, Zinman B
Diabetes Care 2009 32:91-3
Species: Human
Sample Type: Urine
Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
Bang YJ, Choi IS, Han SW, Im SA, Kim MA, Kim TY, Kim WH, Lee KH, Lee KW, Lee MH, Lee NS, Oh DY, Park SR, Song HS
Br. J. Cancer 2009 100:298-304
Species: Human
Sample Type: Serum
A urinary biomarker profile for children with HIV-associated renal diseases.
Mattison PC, Rakhmanina NY, Ray PE, Soler-Garcia AA
Kidney Int. 2009 76:207-14
Species: Human
Sample Type: Urine
Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
Aydin S, Daemen A, De Moor B, Debucquoy A, Gevaert O, Haustermans K, Libbrecht L, Machiels JP, McBride WH, Penninckx F, Scalliet P, Sempoux C, Stroh C, Tejpar S, Vlassak S
J. Clin. Oncol. 2009 27:2751-7
Species: Human
Sample Type: Plasma
Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
Abraham WM, Chen J, Chen T, Forteza RM, Kuang G, Kuzmic P, Martik D, Nixon AE, Sexton DJ, Wood CR, Zuraw BL
Biochem. J. 2009 422:383-92
Species: Human
Sample Type: Cell Culture Supernates
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