Human CCL17/TARC Quantikine ELISA Kit | hCCL17/TARC QKit
掲載日情報：2016/08/18 現在Webページ番号：111073
R&D Systems製のHuman CCL17/TARCを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 17時55分現在]
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TARC, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月26日 17時55分現在]
TARC, Human, ELISA Kit, Quantikine (96well)
文献数： 60
- 商品コード：DDN00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：7 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC22，測定時間：3.5 hours，測定因子：CCL17, TARC 別名：ABCD-2 Genbank No： 6361 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | TARC Quantikine/DuoSet ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 7 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human TARC
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 87 | 600 | 1,107 | 98 | 616 | 1,178 |
| Standard Deviation | 6.2 | 13.7 | 29.9 | 8.9 | 50.7 | 88.8 |
| CV% | 7.1 | 2.3 | 2.7 | 9.1 | 8.2 | 7.5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 88 | 504 | 1,001 | 89 | 540 | 1,033 |
| Standard Deviation | 4.1 | 21.7 | 27.2 | 8.3 | 38.9 | 71.7 |
| CV% | 4.7 | 4.3 | 2.7 | 9.3 | 7.2 | 6.9 |
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Recovery
The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 105 | 100-114 |
| Heparin Plasma (n=5) | 98 | 86-107 |
| EDTA Plasma (n=5) | 102 | 86-111 |
| Cell Culture Media (n=4) | 95 | 88-104 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TARC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CCL17/TARC
- Aliases
- TARC
- Entrez Gene IDs
- 6361 (Human); 20295 (Mouse)
- Background
- CCL17/TARC (Thymus and Activation Regulated Chemokine)
- Thymus and activation-regulated chemokine (TARC) is a novel CC chemokine identified using a signal sequence trap method. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. TARC is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis.
Rai AK, Singh A, Thakur CP
PLoS ONE 2012 7:e31551
Species: Human
Sample Type: Serum
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Adams DH, Curbishley SM, Eksteen B, Harki J, Hubscher SG, Lalor PF, Oo YH, Reynolds GM, Shaw JC, Shetty S, Walker LS, Weston CJ, Withers DR
J. Immunol. 2010 184:2886-98
Species:
Sample Type: Cell Culture Supernates
Mepolizumab as a corticosteroid-sparing agent in lymphocytic variant hypereosinophilic syndrome.
Cogan E, de Lavareille A, Georgelas A, Gleich GJ, Goldman M, Klion A, Raffeld M, Roufosse F, Schandene L, Wagner L, Xi L
J. Allergy Clin. Immunol. 2010 126:828-835
Species: Human
Sample Type: Serum
Brentuximab vedotin (SGN-35) for relapsed CD30-positive lymphomas.
Bartlett NL, Leonard JP, Younes A
N. Engl. J. Med. 2010 363:1812-21
Species: Human
Sample Type: Serum
Increases in serum TARC/CCL17 levels are associated with progression-free survival in advanced melanoma patients in response to dendritic cell-based immunotherapy.
Carbonell DJ, Cornforth AN, Dillman RO, Fowler AW, Lee GJ
J. Clin. Immunol. 2009 29:657-64
Species: Human
Sample Type: Serum
Phenotype of atopic dermatitis subjects with a history of eczema herpeticum.
Barnes KC, Beck LA, Boguniewicz M, Gallo R, Hanifin J, Hata T, Leung DY, Lieff S, Milgrom H, Paller AS, Reese J, Schneider LC, Zaccaro D
J. Allergy Clin. Immunol. 2009 124:260-9, 269.e1
Species: Human
Sample Type: Serum
The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
Bogaczewicz J, Kuna P, Lesiak A, Narbutt J, Stelmach I, Sysa-Jedrzeiowska A, Zakrzewski M
Mediators Inflamm. 2009 2009:269541
Species: Human
Sample Type: Serum
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Miller R, Sadhukhan R, Wu C
J. Immunol. Methods 2008 334:134-41
Species: Human
Sample Type: Cell Culture Supernates
In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
Bruserud ??, Ersvaer E, Olsnes AM, Ryningen A
J. Interferon Cytokine Res. 2008 28:297-310
Species: Human
Sample Type: Cell Culture Supernates
Circulating thymus- and activation-regulated chemokine/CCL17 is a useful biomarker for discriminating acute eosinophilic pneumonia from other causes of acute lung injury.
Ando M, Fukami T, Kumamoto T, Matsuno O, Miyazaki E, Nureki S, Ono E, Ueno T
Chest 2007 131:1726-34
Species: Human
Sample Type: BALF
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