Human PDGF-DD Quantikine ELISA Kit | hPDGF-DD QKit
掲載日情報：2016/08/18 現在Webページ番号：111072
R&D Systems製のHuman PDGF-DDを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月11日 14時15分現在]
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Human PDGF-DD Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月11日 14時15分現在]
Human PDGF-DD Quantikine ELISA Kit
文献数： 1
- 商品コード：DDD00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：3.51 pg/mL，測定範囲：31.30 - 2000 pg/mL，種交差性：Human，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (50 uL), Human Milk (50 uL)，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC140，測定時間：4.5 hours，測定因子：PDGF-DD 別名：PDGFDD Genbank No： 80310 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Featured Product: Human IgE, IgG, & IgM ELISpot B Cell Development Modules |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL), Saliva (50 µL), Human Milk (50 µL)
- Sensitivity
- 3.51 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Saliva, Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human PDGF-DD
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human PDGF-DD Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-DD in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human PDGF-DD and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-DD showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PDGF-DD.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 176 | 510 | 986 | 198 | 559 | 1,095 |
| Standard Deviation | 3.69 | 11.9 | 17.2 | 16 | 38.5 | 55.3 |
| CV% | 2.1 | 2.3 | 1.7 | 8.1 | 6.9 | 5.1 |
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Recovery
The recovery of PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 83-112 |
| Serum (n=4) | 101 | 86-112 |
| Platelet-poor EDTA Plasma (n=4) | 93 | 83-109 |
| Platelet-poor Heparin Plasma (n=4) | 94 | 84-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-DD were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- PDGF-DD
- Aliases
- undefined
- Entrez Gene IDs
- 80310 (Human)
- Background
- PDGF-A, -B, and -D
- The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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