Human Cyr61/CCN1 Quantikine ELISA Kit | hCyr61 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111071
R&D Systems製のHuman Cyr61/CCN1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月22日 00時00分現在]
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Human Cyr61/CCN1 Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月22日 00時00分現在]
Human Cyr61/CCN1 Quantikine ELISA Kit
文献数： 3
- 商品コード：DCYR10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human，感度：3.8 pg/mL，測定範囲：39.10 - 2500 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Saliva (50 uL), Human Milk (10 uL)，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC73，測定時間：4.5 hours，測定因子：Cyr61/CCN1 別名：CCN family member 1 Genbank No： 3491 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human Cyr61 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Saliva (50 µL), Human Milk (10 µL)
- Sensitivity
- 3.8 pg/mL
- Assay Range
- 39.1 - 2,500 pg/mL (Serum, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human Cyr61
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 256 | 721 | 1,442 | 249 | 712 | 1,445 |
| Standard Deviation | 5.94 | 14.2 | 33.5 | 15.9 | 35 | 73.3 |
| CV% | 2.3 | 2 | 2.3 | 6.4 | 4.9 | 5.1 |
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Recovery
The recovery of Cyr61 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 107 | 98-117 |
| Serum (n=4) | 101 | 95-113 |
| EDTA Plasma (n=4) | 98 | 91-104 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cyr61 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Cyr61/CCN1
- Aliases
- CCN1; GIG1; IGFBP-10
- Entrez Gene IDs
- 3491 (Human); 16007 (Mouse); 83476 (Rat)
- Background
- Cyr61
- Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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