Human Cystatin B Quantikine ELISA Kit | hCystatin B Qkit
掲載日情報：2016/08/18 現在Webページ番号：111070
R&D Systems製のHuman Cystatin Bを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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Human Cystatin B Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月05日 00時00分現在]
Human Cystatin B Quantikine ELISA Kit
文献数： 0
- 商品コード：DCYB00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human，感度：0.034 ng/mL，測定範囲：0.16 - 10 ng/mL，測定試料：Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (10 uL), Urine (13 uL), Human Milk (10 uL)，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：#QC74，測定時間：4.5 hours，測定因子：Cystatin B 別名：CPI-B Genbank No： 1476 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human Cystatin B ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL), Saliva (10 µL), Urine (13 µL), Human Milk (10 µL)
- Sensitivity
- 0.034 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Cystatin B
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Cystatin B Immunoassay is a 4.5 hour solid phase ELISA designed to measure Cystatin B levels in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Cystatin B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Cystatin B and recombinant human Cystatin B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Cystatin B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Cystatin B.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.38 | 2.73 | 5.47 | 1.42 | 2.78 | 5.4 |
| Standard Deviation | 0.049 | 0.082 | 0.164 | 0.148 | 0.191 | 0.263 |
| CV% | 3.6 | 3 | 3 | 10.4 | 6.9 | 4.9 |
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Recovery
The recovery of Cystatin B spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 90-115 |
| Serum (n=4) | 93 | 83-103 |
| EDTA Plasma (n=4) | 96 | 86-103 |
| Heparin Plasma (n=4) | 90 | 81-97 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of Cystatin B were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Cystatin B
- Aliases
- CSTB; Stefin B
- Entrez Gene IDs
- 1476 (Human); 13014 (Mouse)
- Background
- Cystatin B
- Cystatin B, also known as Stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily. It is a 98 amino acid, intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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