Human CXCL9/MIG Quantikine ELISA Kit | hCXCL9/MIG Qkit
掲載日情報：2016/08/18 現在Webページ番号：111069
R&D Systems製のHuman CXCL9/MIGを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月28日 00時00分現在]
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MIG, Human, ELISA Kit, Quantikine (96 well) <CXCL9, ELISA Kit> |
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[在庫・価格 ：2024年04月28日 00時00分現在]
MIG, Human, ELISA Kit, Quantikine (96 well) <CXCL9, ELISA Kit>
文献数： 40
- 商品コード：DCX900
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：11.3 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC123，測定時間：4.5 hours，測定因子：CXCL9/MIG 別名：chemokine (C-X-C motif) ligand 9 Genbank No： 4283 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
CXCL9/MIG Quantikine/DuoSet ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 11.3 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human MIG
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 253 | 817 | 1,603 | 245 | 723 | 1,427 |
| Standard Deviation | 9.9 | 26.6 | 49.6 | 12.8 | 59.4 | 88.1 |
| CV% | 3.9 | 3.3 | 3.1 | 5.2 | 8.2 | 6.2 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 235 | 700 | 1,358 | 242 | 707 | 1,391 |
| Standard Deviation | 9.5 | 25.1 | 24.3 | 20.8 | 50.9 | 83.6 |
| CV% | 4 | 3.6 | 1.8 | 8.6 | 7.2 | 6 |
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Recovery
The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 108 | 101-113 |
| Heparin Plasma (n=5) | 109 | 101-114 |
| EDTA Plasma (n=5) | 100 | 95-110 |
| Cell Culture Media (n=4) | 92 | 86-98 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of MIG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL9/MIG
- Aliases
- MIG
- Entrez Gene IDs
- 4283 (Human); 17329 (Mouse); 246759 (Rat)
- Background
- CXCL9/MIG
- MIG, a member of the CXC subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. MIG is a chemoattractant for activated T cells and TIL, but not for neutrophils or monocytes.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
Grandel B, Knoblach T, Nevels M, Paulus C, Seiler J
PLoS Pathog. 2011 7:e1002016
Species: Human
Sample Type: Cell Culture Supernates
Circulating chemokine (CXC motif) ligand (CXCL)9 is increased in aggressive chronic autoimmune thyroiditis, in association with CXCL10.
Antonelli A, Benvenga S, Corrado A, Fallahi P, Ferrannini E, Ferrari SM, Franzoni F, Frascerra S, Galetta F, Miccoli M, Paolicchi A
Cytokine 2011 55:288-93
Species: Human
Sample Type: Serum
Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
Choi HJ, Im SK, Lee KH, Park CS, Park HY, Pflugfelder SC, Yoon KC, You IC
Invest. Ophthalmol. Vis. Sci. 2010 51:643-50
Species: Human
Sample Type: Tears
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Chhauy S, Draghici S, Gotsch F, Hassan SS, Kim CJ, Kim JS, Kim MJ, Kusanovic JP, LaJeunesse C, Lee DC, Romero R, Tarca AL
J. Immunol. 2009 182:3919-27
Species: Human
Sample Type: Plasma
CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
Belperio JA, Burdick MD, Busuttil A, Elashoff RM, Fishbein M, Huang C, Keane MP, Lynch JP, Palchevskiy V, Strieter RM, Weigt SS, Xue YY, Zisman DA
Eur. Respir. J. 2009 34:676-86
Species: Human
Sample Type: BALF
The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
Bogaczewicz J, Kuna P, Lesiak A, Narbutt J, Stelmach I, Sysa-Jedrzeiowska A, Zakrzewski M
Mediators Inflamm. 2009 2009:269541
Species: Human
Sample Type: Serum
The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
Bjartell A, Collin M, Egesten A, Giwercman A, Linge HM, Malm J
J. Interferon Cytokine Res. 2008 28:191-6
Species: Human
Sample Type: seminal plasma
Urinary fractalkine is a marker of acute rejection.
Chen J, Chen Y, He Q, Jiang Y, Peng W, Shou Z, Wang H, Wang Y, Wu J
Kidney Int. 2008 74:1454-60
Species: Human
Sample Type: Urine
IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
Dame TM, Furie MB, Orenzoff BL, Palmer LE
J. Immunol. 2007 178:1172-9
Species: Human
Sample Type: Cell Culture Supernates
Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
Blotta MH, Corvino CL, Fagundes GZ, Mamoni RL
Clin. Exp. Immunol. 2007 147:483-90
Species: Human
Sample Type: Serum
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