Human CX3CL1/Fractalkine Quantikine ELISA Kit | hFractalkine Qkit
掲載日情報：2016/08/18 現在Webページ番号：111068
R&D Systems製のHuman CX3CL1/Fractalkineを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月27日 20時55分現在]
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Fractalkine, Human, ELISA Kit, Quantikine (96well) <CX3CL1, ELISA Kit> |
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[在庫・価格 ：2024年05月27日 20時55分現在]
Fractalkine, Human, ELISA Kit, Quantikine (96well) <CX3CL1, ELISA Kit>
文献数： 21
- 商品コード：DCX310
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.072 ng/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (50 uL), Urine (50 uL), Human Milk (100 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC93，測定時間：4.5 hours，測定因子：CX3CL1/Fractalkine 別名：ABCD-3 Genbank No： 6376 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2018年7月1日号 p.11 ニュース2019年8月15日号 p.15
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| 製品記事 |
がん幹細胞関連因子測定用 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (50 µL), Urine (50 µL), Human Milk (100 µL)
- Sensitivity
- 0.072 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Fractalkine. This assay detects all forms of human Fractalkine containing the chemokine domain.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.24 | 3.8 | 7.12 | 1.23 | 3.59 | 6.88 |
| Standard Deviation | 0.04 | 0.08 | 0.17 | 0.11 | 0.2 | 0.37 |
| CV% | 3.2 | 2.1 | 2.4 | 8.9 | 5.6 | 5.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.18 | 3.33 | 6.43 | 1.19 | 3.53 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.24 | 0.07 | 0.15 | 0.31 |
| CV% | 2.5 | 3.4 | 3.7 | 6.1 | 4.3 | 4.5 |
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Recovery
The recovery of Fractalkine spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 99 | 88-111 |
| Heparin Plasma (n=4) | 97 | 85-108 |
| EDTA Plasma (n=4) | 101 | 88-113 |
| Urine (n=4) | 96 | 86-109 |
| Cell Culture Media (n=4) | 98 | 93-102 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Fractalkine were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CX3CL1/Fractalkine
- Aliases
- FKN; Fractalkine; Neurotactin
- Entrez Gene IDs
- 6376 (Human); 20312 (Mouse); 89808 (Rat)
- Background
- CX3CL1/Fractalkine
- Fractalkine, also named neurotactin, is a chemokine identified through bioinformatics. Fractalkine has a unique CX3C cysteine motif near the amino-terminus and is the one member of a fourth branch of the chemokine superfamily. The extracellular domain of human Fractalkine can be released by proteolysis, at the dibasic cleavage site proximal to the membrane to generate soluble fractalkine.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Identification of the chemokine CX3CL1 as a new regulator of malignant cell proliferation in epithelial ovarian cancer.
Balabanian K, Combadiere C, Donnadieu AC, Emilie D, Gaudin F, Machelon V, Nasreddine S, Prevot S
PLoS ONE 2012 6:e21546
Species: Human
Sample Type: Cell Culture Supernates
Altered expression of CX3CL1 in patients with epilepsy and in a rat model.
Chen D, Chen G, Han Y, Wang H, Wang L, Wang X, Xi Z, Xu Y, Zeng K
Am. J. Pathol. 2012 180:1950-62
Species: Human
Sample Type: CSF
Fractalkine is expressed in early and advanced atherosclerotic lesions and supports monocyte recruitment via CX3CR1.
Barocke V, Bauersachs J, Bray P, Heider P, Lorenz M, Massberg S, Pelisek J, Schafer A, Schulz C, Steinhart A, Stolla M, Tirniceriu A, von Bruhl M
PLoS ONE 2012 7:e43572
Species: Human
Sample Type: Serum
Early detection of malignant pleural mesothelioma in asbestos-exposed individuals with a noninvasive proteomics-based surveillance tool.
Ayers D, Black B, Brody E, Carbone M, Goparaju C, Harbut M, Levin S, Mehan M, Ostroff R, Pass H, Stewart A, Williams S
PLoS ONE 2012 7:e46091
Species: Human
Sample Type: Serum
Activated innate immunity and the involvement of CX3CR1-fractalkine in promoting hematuria in patients with IgA nephropathy.
Kidney Int. 2012 82:548-60
Species: Human
Sample Type: Urine
Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis 2012 12:144
Species: Human
Sample Type: Serum
Fractalkine is an independent predictor of mortality in patients with advanced heart failure.
Thromb Haemost 2012 108:1220-7
Species: Human
Sample Type: Plasma
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Ahima RS, Ferguson JF, Hinkle CC, Li M, Lu Y, Mehta NN, Putt ME, Qu L, Reilly MP, Shah R
Diabetes 2011 60:1512-8
Species: Human
Sample Type: Plasma
Characterization of synergistic induction of CX3CL1/fractalkine by TNF-alpha and IFN-gamma in vascular endothelial cells: an essential role for TNF-alpha in post-transcriptional regulation of CX3CL1.
Imaizumi T, Matsumiya T, Ota K
J. Immunol. 2010 184:4205-14
Species: Human
Sample Type: Cell Culture Supernates
Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
Chaukos D, Crow MR, Huang YW, Liu GY, Robinson LA, Su P, Yan H
J. Biol. Chem. 2009 284:29644-53
Species: Human
Sample Type: Cell Culture Supernates
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