Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit | hBLC/BCA-1 Qkit

R&D Systems製のHuman CXCL13/BLC/BCA-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。

The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural BLC/BCA-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
Bisser S, Buscher P, Courtioux B, Enyaru JC, Hainard A, Krishna S, Kristensson K, Lejon V, Matovu E, Ndung'u JM, Ngoyi DM, Robin X, Sanchez JC, Tiberti N, Turck N, Vatunga GM
PLoS ONE 2012 7:e40909
Species: Human
Sample Type: CSF


The chemokine CXCL13 is a prognostic marker in clinically isolated syndrome (CIS).
Brettschneider J, Czerwoniak A, Senel M
PLoS ONE 2010 5:e11986
Species: Human
Sample Type: Serum


Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Chhauy S, Draghici S, Gotsch F, Hassan SS, Kim CJ, Kim JS, Kim MJ, Kusanovic JP, LaJeunesse C, Lee DC, Romero R, Tarca AL
J. Immunol. 2009 182:3919-27
Species: Human
Sample Type: Plasma


Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
Chung LW, Cooper CR, Grizzle WE, Lillard JW, Novakovic KR, Rai SN, Sharma PK, Singh R, Singh S, Singh UP
Cancer Lett. 2009 283:29-35
Species: Human
Sample Type: Serum


Potential novel biomarkers of disease activity in rheumatoid arthritis patients: CXCL13, CCL23, transforming growth factor alpha, tumor necrosis factor receptor superfamily member 9, and macrophage colony-stimulating factor.
Akil M, Binks MH, Dickson MC, Hughes FJ, Montgomery DS, Rioja I, Sharp CH, Warnock LC, Wilson AG
Arthritis Rheum. 2008 58:2257-67
Species: Human
Sample Type: Plasma


Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
Atanackovic D, Bartels K, Bokemeyer C, Cao Y, Friedrichs K, Hegewisch-Becker S, Hildebrandt Y, Horn C, Kroger N, Leuwer R, Luetkens T, Marx A, Milde-Langosch K, Niendorf A, Panse J, Stahl T, Wenzel S
Br. J. Cancer 2008 99:930-8
Species: Human
Sample Type: Serum


Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis.
Conteduca V, Dammacco F, Lauletta G, Montrone M, Sansonno D, Sansonno L, Troiani L, Tucci FA
Blood 2008 112:1620-7
Species: Human
Sample Type: Serum


Lymphoid chemokines in chronic neuroinflammation.
Aloisi F, Ambrosini E, Coccia E, Columba-Cabezas S, Franciotta D, Magliozzi R, Reynolds R, Rosicarelli B, Salvetti M, Serafini B
J. Neuroimmunol. 2008 198:106-12
Species: Human
Sample Type: CSF


Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Atlas A, Cagigi A, Chiodi F, Grutzmeier S, Mowafi F, Nilsson A, Phuong Dang LV, Racz P, Tenner-Racz K
Blood 2008 112:4401-10
Species: Human
Sample Type: Cell Culture Supernates


High levels of inflammatory chemokines and cytokines in joint fluid and synovial tissue throughout the course of antibiotic-refractory lyme arthritis.
Glickstein LJ, Shin JJ, Steere AC
Arthritis Rheum. 2007 56:1325-35
Species: Human
Sample Type: Synovial Fluid