Human Clusterin Quantikine ELISA Kit | hClusterin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111058
R&D Systems製のHuman Clusterinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月17日 09時55分現在]
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Clusterin, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年07月17日 09時55分現在]
Clusterin, Human, ELISA Kit, Quantikine (96well)
文献数： 11
- 商品コード：DCLU00
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：3.12-200 ng/mL，感度：1.05 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC64，測定時間：4.5 hours，測定因子：Clusterin 別名：40 Genbank No： 1191 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Clusterin Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (25 µL), Urine (13 µL)
- Sensitivity
- 1.05 ng/mL
- Assay Range
- 3.12 - 200 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Clusterin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Clusterin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Clusterin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Clusterin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Clusterin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Clusterin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 19 | 64.3 | 130 | 22.1 | 64.1 | 136 |
| Standard Deviation | 0.7 | 2.4 | 4.5 | 1.6 | 5.4 | 9.3 |
| CV% | 3.7 | 3.7 | 3.5 | 7.2 | 8.4 | 6.8 |
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Recovery
The recovery of Clusterin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 107 | 102-110 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of Clusterin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Clusterin
- Aliases
- APOJ; CLI; CLU; SGP-2; SP-40; TRPM-2
- Entrez Gene IDs
- 1191 (Human); 12759 (Mouse)
- Background
- Clusterin
- Clusterin (named for its ability to aggregate, or cluster, cells) is an 80-kDa, secreted, heterodimeric glycoprotein that has no identifiable structural motif(s). It is generated from a common precursor that is cleaved to form two disulfide-linked subunits. Clusterin is believed to function as a unique type of heat shock protein, presumably protecting extracellular molecules. Alternative splicing will also generate a truncated form of Clusterin that is pro-apoptotic. Thus, its function(s) may be context-dependent.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Semen clusterin is a novel DC-SIGN ligand.
Amigorena S, Arenzana-Seisdedos F, Ceballos A, Faigle W, Fernandez M, Fieschi F, Geffner J, Lenicov FR, Lortat-Jacob H, Malchiodi E, Michalski JC, Morelle W, Rodr?gues CR, Sabatte J, Thepaut M
J. Immunol. 2011 187:5299-309
Species: Human
Sample Type: seminal plasma
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