Human ICAM-1/CD54 Non Allele-specific Quantikine ELISA Kit | hICAM-1 Non Allele-spec Qkit
掲載日情報：2016/08/18 現在Webページ番号：111055
R&D Systems製のHuman ICAM-1/CD54 Non Allele-specificを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
ICAM-1/CD54, Human, Non Allele-specific, ELISA Kit, Quantikine |
|
1 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月29日 20時55分現在]
ICAM-1/CD54, Human, Non Allele-specific, ELISA Kit, Quantikine
文献数： 1
- 商品コード：DCIM00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，測定範囲：0.625-40 ng/mL，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 ewll，検出方法：呈色，種交差性：Human，別売品コントロール：#QC188，感度：0.053 ng/mL，測定時間：2.5 hours，測定因子：ICAM-1, CD54 Non Allele-specific 別名：BB2 Genbank No： 3383 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
|
||
| 製品記事 | Human ICAM-1 / CD54 Non Allele-specific Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
||
| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 0.053 ng/mL
- Assay Range
- 0.625 - 40 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human ICAM-1
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human ICAM-1/CD54 Non Allele-specific Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human ICAM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human ICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ICAM-1.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 3.03 | 9.64 | 20 | 3.27 | 9.81 | 19.5 |
| Standard Deviation | 0.128 | 0.346 | 0.973 | 0.282 | 0.543 | 1.22 |
| CV% | 4.2 | 3.6 | 4.9 | 8.6 | 5.5 | 6.3 |
追加しました。
Recovery
The recovery of human ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 96-117 |
| Urine (n=4) | 94 | 85-106 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ICAM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- ICAM-1/CD54
- Aliases
- CD54
- Entrez Gene IDs
- 3383 (Human); 15894 (Mouse); 25464 (Rat)
- Background
- ICAM-1/CD54
- Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。