Human Coagulation Factor III/Tissue Factor Quantikine ELISA | hFactor III/TF QKit
掲載日情報：2016/08/18 現在Webページ番号：111053
R&D Systems製のHuman Coagulation Factor III/Tissue Factor Quantikine ELISAを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
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Coagulation Factor III, Human, ELISA Kit, Quantikine (96well) <Tissue Factor, ELISA Kit> |
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[在庫・価格 ：2025年04月29日 20時55分現在]
Coagulation Factor III, Human, ELISA Kit, Quantikine (96well) <Tissue Factor, ELISA Kit>
文献数： 19
- 商品コード：DCF300
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：7.8-500 pg/mL，感度：2.05 pg/mL，測定試料：Plasma, Cell Culture Supernatant, Urine, Tissue Lysate，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC65，測定時間：4.5 hours，測定因子：Coagulation Factor III, Tissue Factor 別名：CD142 Genbank No： 2152 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Coagulation Factor III/ Tissue Factor Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL), Urine (50 µL)
- Sensitivity
- 2.05 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Heparin Plasma, Cell Culture Supernates, Cell Lysates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Coagulation Factor III
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 33.4 | 65.4 | 148 | 71 | 144 | 301 |
| Standard Deviation | 0.78 | 1.81 | 5 | 4.5 | 7.8 | 17.1 |
| CV% | 2.3 | 2.8 | 3.4 | 6.3 | 5.4 | 5.7 |
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Recovery
The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Heparin Plasma (n=4) | 95 | 88-109 |
| Citrate Plasma (n=4) | 92 | 86-102 |
| EDTA Plasma (n=4) | 91 | 86-97 |
| Urine (n=4) | 102 | 96-112 |
| Cell Culture Media (n=4) | 104 | 95-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Coagulation Factor III/Tissue Factor
- Aliases
- CD142; F3; Thromboplastin; Tissue Factor
- Entrez Gene IDs
- 2152 (Human); 14066 (Mouse)
- Background
- Coagulation Factor III/Tissue Factor
- Coagulation Factor III/Tissue Factor (TF), also known as Thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of coagulation factor VII, which is synthesized in the liver and circulated in the plasma. Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1 to 32) and the mature chain (residues 33 to 295), which includes a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 295).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Tissue factor-bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo.
Drazen J, Howarth P, Lau L, Limbrey R, Park J, Sharif A, Tschumperlin D
J Allergy Clin Immunol 2012 130:1375-83
Species: Human
Sample Type: Cell Culture Supernates
Toward engineering a human neoendothelium with circulating progenitor cells.
Allen JB, Ameer GA, Khan S, Lapidos KA
Stem Cells 2010 28:318-28
Species: Human
Sample Type: Cell Lysates
Traditional risk factors and D-dimer predict incident cardiovascular disease events in chronic HIV infection.
Ford ES, Greenwald JH, Richterman AG
AIDS 2010 24:1509-17
Species: Human
Sample Type: Plasma
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