Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit | hICAM-1 Allele-specific Qkit
掲載日情報：2016/08/18 現在Webページ番号：111050
R&D Systems製のHuman ICAM-1/CD54 Allele-specificを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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Human sICAM-1/CD54 Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月05日 00時00分現在]
Human sICAM-1/CD54 Quantikine ELISA Kit
文献数： 64
- 商品コード：DCD540
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.254 ng/mL，測定範囲：1.56 - 50 ng/mL，種交差性：Human，測定試料：Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC105，測定時間：2 hours，測定因子：ICAM-1/CD54 別名：BB2 Genbank No： 3383 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
R&D Systems 社 sICAM-1/CD54関連製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.254 ng/mL
- Assay Range
- 1.56 - 50 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human sICAM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human sICAM-1 immunoassay is a 2.0 hour solid phase ELISA designed to measure sICAM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human sICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sICAM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 4.61 | 12.1 | 19.6 | 4.77 | 12.5 | 20.5 |
| Standard Deviation | 0.17 | 0.63 | 0.98 | 0.32 | 0.67 | 0.9 |
| CV% | 3.7 | 5.2 | 5 | 6.7 | 5.4 | 4.4 |
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Recovery
The recovery of sICAM-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 104 | 90-109 |
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Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of sICAM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- ICAM-1/CD54
- Aliases
- CD54
- Entrez Gene IDs
- 3383 (Human); 15894 (Mouse); 25464 (Rat)
- Background
- ICAM-1/CD54
- Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
Carrero JJ, Davidge ST, Hammarqvist F, Kublickiene K, Luksha L, Stenvinkel P
PLoS ONE 2012 7:e36056
Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
Chang CS, Huang CS, Kuo CL, Liu CS, Su SL, Wu SL, Yu SC
Biomarkers 2012 17:319-24
Species: Human
Sample Type: Plasma
Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
Bisser S, Buscher P, Courtioux B, Enyaru JC, Hainard A, Krishna S, Kristensson K, Lejon V, Matovu E, Ndung'u JM, Ngoyi DM, Robin X, Sanchez JC, Tiberti N, Turck N, Vatunga GM
PLoS ONE 2012 7:e40909
Species: Human
Sample Type: CSF
Suppression of NF-kappaB Reduces Myocardial No-Reflow.
Chen Y, Liu C, Lv Y, Yan H, Zeng M, Zhao B, Zhao H, Zhou P
PLoS ONE 2012 7:e47306
Species: Human
Sample Type: Cell Culture Supernates
Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis 2012 12:144
Plasma markers for identifying patients with metastatic melanoma.
Ariyan S, Bacchiocchi A, Halaban R, Hoyt K, Kirsch J, Kluger HM, Kluger Y, Mayer T, Molinaro A, Sznol M
Clin. Cancer Res. 2011 17:2417-25
Species: Human
Sample Type: Plasma
Divergent anti-inflammatory effects of different oil acute consumption on healthy individuals.
Antoniades C, Giolis A, Miliou A, Papageorgiou N, Psaltopoulou T, Siasos G, Stefanadis C, Tousoulis D, Toutouzas K, Tsiamis E
Eur J Clin Nutr 2011 65:514-9
Species: Human
Sample Type: Serum
Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
Blaak EE, Defoort C, Dembinska-Kiec A, Drevon CA, Gibney MJ, Golabek-Leszczynska I, Gulseth HL, Helal O, Karsltrom B, Lopez-Miranda J, Lovegrove JA, McMonagle J, Paniagua JA, Roche HM, Saris WH, Shaw DI, Tierney AC, Vessby B, Williams CM
Int J Obes (Lond) 2011 35:800-9
Species: Human
Sample Type: Serum
Prognostic significance of angiogenic factors in uterine cervical cancer.
Blohmer JU, Heidecke H, Jeschke S, Korlach S, Kummel S, Landt S, Lichtenegger W, Schmid P, Sehouli J, Stoblen F, Wehling M
Anticancer Res. 2011 31:2589-95
Species: Human
Sample Type: Serum
Influence of a dose-dense adjuvant chemotherapy on sVCAM-1/sICAM-1 serum levels in breast cancer patients with 1-3 positive lymph nodes.
Eggeman H, Elling D, Korlach S, Kummel S, Landt S, Luftner D, Schmid P, Stoblen F, Taran FA, Thill M
Anticancer Res. 2011 31:2617-22
Species: Human
Sample Type: Serum
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