Human Calbindin D Quantikine ELISA Kit | hCalbindin D QKit
掲載日情報：2016/08/18 現在Webページ番号：111041
R&D Systems製のHuman Calbindin Dを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Human Calbindin D Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Human Calbindin D Quantikine ELISA Kit
文献数： 0
- 商品コード：DCALD0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 試料に含まれる天然型または組換え体Calbindin Dを，サンドイッチELISAにより比色定量するキット。別売品コントロール：QC189，感度：0.015 ng/mL，測定範囲：0.078-5 ng/mL，測定試料：Urine, Tissue Lysate，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：Calbindin D 別名：CABP, CABP1, CABP28K, CABP9K, Calbindin 3, Calbindin-D, Calbindin-D9K, S100G Genbank No： 795 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Human Calbindin D Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Tissue Lysates (10 µL), Urine (25 µL)
- Sensitivity
- 0.015 ng/mL
- Assay Range
- 0.078 - 5 ng/mL
- Specificity
- Natural and recombinant human Calbindin D.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Calbindin D Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Calbindin D in tissue lysates and urine. It contains E. coli-expressed recombinant human Calbindin D and antibodies raised against the recombinant factor. Natural human Calbindin D showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural human Calbindin D.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Tissue Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.511 | 1.65 | 2.84 | 0.548 | 1.57 | 2.96 |
| Standard Deviation | 0.026 | 0.04 | 0.072 | 0.038 | 0.075 | 0.15 |
| CV% | 5.1 | 2.4 | 2.5 | 6.9 | 4.8 | 5.1 |
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Recovery
The recovery of human Calbindin D spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Urine (n=4) | 93 | 89-98 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human Calbindin D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- Calbindin D
- Aliases
- CABP1; CABP9K; S100G
- Entrez Gene IDs
- 795 (Human); 12309 (Mouse); 24249 (Rat)
- Background
- Calbindin D
- Calbindin D is a highly conserved vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin-D is widely expressed and regulates calcium homeostasis. Calbindin-D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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