Human Chitinase 3-like 1 Quantikine ELISA Kit | hCHI3L1 Qkit

掲載日情報:2016/08/18 現在Webページ番号:111035

R&D Systems製のHuman Chitinase 3-like 1を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年03月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
CHI3L1, Human, ELISA Kit, Quantikine (96well) <Chitinase 3-Like 1, ELISA Kit>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 31
説明文
測定範囲:62.50 - 4000 pg/mL,感度:8.15 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC63,測定時間:4.5 hours,測定因子:Chitinase 3-like 1
別名:AW208766
Genbank No: 1116
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Chitinase 3-like 1/CHI3L1 Quantikine/DuoSet ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年03月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

CHI3L1, Human, ELISA Kit, Quantikine (96well) <Chitinase 3-Like 1, ELISA Kit>

文献数: 31

説明文 測定範囲:62.50 - 4000 pg/mL,感度:8.15 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC63,測定時間:4.5 hours,測定因子:Chitinase 3-like 1
別名:AW208766
Genbank No: 1116
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Chitinase 3-like 1/CHI3L1 Quantikine/DuoSet ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity
8.15 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
Specificity
Natural and recombinant human CHI3L1
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 566 1,031 2,202 606 1,019 2,212
Standard Deviation 26.7 44.4 102.6 32.1 59.1 153.1
CV% 4.7 4.3 4.7 5.3 5.8 6.9

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Recovery

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 105 90-115
Cell Culture Media (n=4) 97 93-100

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Linearity

To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
Chitinase 3-like 1
Aliases
CHI3L1; HCgp39; YKL-40
Entrez Gene IDs
1116 (Human); 12654 (Mouse)
Background
Chitinase 3-like 1
CHI3L1, also known as HCgp39 (human cartilage glycoprotein 39) and YKL40, is a secreted glycoprotein belonging to the family of chitinase-like lectins. These proteins are structurally related to the glycosylhydrolase family 18, but lack enzymatic activity. CHI3L1 is expressed by chondrocytes, synovial cells, macrophages and neutrophils. It has been found to play a role in down-regulating cytokine-induced inflammatory responses.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Levels of YKL-40 in pleural effusions and blood from patients with pulmonary or pleural disease.
Cho JH, Jeong ET, Jun CD, Kim HR, Lee KS, Lee YJ, Park DS, Yang SH
Cytokine 2012 58:336-43
Species: Human
Sample Type: Serum


The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
Bohla A, Casaulta C, Eber E, Eickelberg O, Eickmeier O, Griese M, Hartl D, Hector A, Kappler M, Kieninger E, Koller B, Kormann MS, Latzin P, Mack I, Mall MA, Rieber N, Yildirim A??, Zhou Z, Zielen S
PLoS ONE 2011 6:e24399
Species: Human
Sample Type: Serum


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