Human CD163 Quantikine ELISA Kit | hCD163 QKit
掲載日情報：2016/08/18 現在Webページ番号：111034
R&D Systems製のHuman CD163を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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CD163, Human, ELISA Kit, Quantikine(96 well) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
CD163, Human, ELISA Kit, Quantikine(96 well)
文献数： 26
- 商品コード：DC1630
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：ご照会下さい ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：1.56-100 ng/mL，感度：0.613 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC61，測定時間：4.5 hours，測定因子：CD163 別名：CD163 Genbank No： 9332 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R&D Systems社 CD163関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.613 ng/mL
- Assay Range
- 1.56 - 100 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant human CD163
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 20 | 35.1 | 65.6 | 20 | 34.9 | 63.6 |
| Standard Deviation | 0.75 | 1.2 | 2.3 | 1.3 | 1.6 | 2.6 |
| CV% | 3.8 | 3.4 | 3.5 | 6.7 | 4.6 | 4.1 |
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Recovery
The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 107 | 101-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD163 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CD163
- Aliases
- CD163; GHI/61; HbSR; M130; RM3/1
- Entrez Gene IDs
- 9332 (Human); 93671 (Mouse); 312701 (Rat)
- Background
- CD163
- CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
Shimizu M, Yachie A
Cytokine 2012 60:226-32
Species: Human
Sample Type: Serum
Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Blanco-Colio LM, Dejouvencel T, Dussiot M, Egido J, Emmerich J, Gaussem P, Labreuche J, Martin-Ventura JL, Meilhac O, Michel JB, Moreno JA, Smadja DM
Arterioscler. Thromb. Vasc. Biol. 2010 30:1253-62
Species: Human
Sample Type: Plasma
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