Human sCD14 Quantikine ELISA Kit | hsCD14 QKit
掲載日情報：2016/08/18 現在Webページ番号：111033
R&D Systems製のHuman sCD14を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月02日 20時35分現在]
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CD14, Soluble, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年05月02日 20時35分現在]
CD14, Soluble, Human, ELISA Kit, Quantikine (96 well)
文献数： 122
- 商品コード：DC140
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：250.00 - 16000 pg/mL，感度：125 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：CD14 別名：CD14 Genbank No： 929 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human sCD14 / Mouse CD14 ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
- Sensitivity
- 125 pg/mL
- Assay Range
- 250 - 16,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human sCD14
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1,111 | 2,158 | 4,187 | 1,159 | 2,298 | 4,346 |
| Standard Deviation | 71 | 104 | 216 | 86 | 153 | 209 |
| CV% | 6.4 | 4.8 | 5.2 | 7.4 | 6.7 | 4.8 |
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Recovery
The recovery of sCD14 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 98 | 84-107 |
| Heparin Plasma (n=5) | 96 | 91-104 |
| Citrate Plasma (n=5) | 102 | 88-110 |
| EDTA Plasma (n=5) | 98 | 90-107 |
| Cell Culture Media (n=5) | 91 | 85-105 |
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Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of sCD14 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CD14
- Aliases
- CD14
- Entrez Gene IDs
- 929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
- Background
- CD14
- CD14 is a 55 kDa GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The amino acid sequence of mature human CD14 is approximately 67% and 64% identical to the mouse and rat proteins, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Altered monocyte activation markers in Tourette's syndrome: a case-control study.
Dehning S, Gruber R, Krause DL, Matz J, Muller N, Riedel M, Schwarz MJ
BMC Psychiatry 2012 12:29
Species: Human
Sample Type: Serum
Relationship between microbial translocation and endothelial function in HIV infected patients.
Aldrovandi G, Blodget E, Dube M, Gupta S, Rollie A, Shen C, Stein J
PLoS ONE 2012 7:e42624
Species: Human
Sample Type: Plasma
CD4 T follicular helper cell dynamics during SIV infection.
J. Clin. Invest. 2012 122:3281-94
Species: Primate - Macaca mulatta (Rhesus monkey)
Sample Type: Plasma
Therapeutic helminth infection of macaques with idiopathic chronic diarrhea alters the inflammatory signature and mucosal microbiota of the colon.
Ardeshir , Amir, Broadhurst , Mara Jan, Gundra , Uma Mahe, Kanwar , Bittoo, Kim , Charlie , Lerche , Nicholas, Leung , Jacqueli, McCune , Joseph M, Mirpuri , Julie, Vujkovic-Cvijin , Ivan, Wiens , Kirsten , Yarovinsky , Felix
Oncogene 2012 8:e1003000
Species: Human
Sample Type: Plasma
Shared monocyte subset phenotypes in HIV-1 infection and in uninfected subjects with acute coronary syndrome.
Costa , Marco A, Funderburg , Nicholas, Lederman , Michael , Lioi , Anthony, Mudd , Joseph, Musselwhite , Laura W, Rodriguez , Benigno, Shive , Carey, Sieg , Scott F, Simon , Daniel I, Zidar , David A
Blood 2012 120:4599-608
Species: Human
Sample Type: Plasma
Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
Altfeld M, Anahtar MN, Brumme Z, Caron T, Chang JJ, Chevalier MF, Flanders M, Jolin JS, Julg B, Kaufmann DE, Kwon DS, Law K, Lian J, Milner DA, Piechoka-Trocha A, Pyo A, Rodig SJ, Streeck H, Toth I, Trocha K, Walker BD
Blood 2011 117:5112-22
Species: Human
Sample Type: Plasma
Increased tissue factor expression on circulating monocytes in chronic HIV infection: relationship to in vivo coagulation and immune activation.
Funderburg NT, Mayne E, Sieg SF
Blood 2010 115:161-7
Species: Human
Sample Type: Plasma
Traditional risk factors and D-dimer predict incident cardiovascular disease events in chronic HIV infection.
Ford ES, Greenwald JH, Richterman AG
AIDS 2010 24:1509-17
Species: Human
Sample Type: Plasma
Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
Amoudruz P, Doumbo O, Doumbo S, Haileselassie Y, Holmlund U, Johansson MA, Kayentao K, Montgomery SM, Ongoiba A, Schollin J, Sverremark-Ekstrom E, Traore B
Clin. Exp. Immunol. 2010 162:500-9
Species: Human
Sample Type: Milk
Ethnic and sex differences in circulating endotoxin levels: A novel marker of atherosclerotic and cardiovascular risk in a British multi-ethnic population.
Alberti KG, Cappuccio FP, Harte AL, Kumar S, McTernan PG, Miller MA, Silva NF, Strazzullo P
Atherosclerosis 2009 203:494-502
Species: Human
Sample Type: Plasma
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