Human BMP-4 Quantikine ELISA Kit | hBMP-4 QKit
掲載日情報：2016/08/18 現在Webページ番号：111031
R&D Systems製のHuman BMP-4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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BMP-4, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
BMP-4, Human, ELISA Kit, Quantikine (96 well)
文献数： 16
- 商品コード：DBP400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：3.68 pg/mL，測定試料：Cell Culture Supernatant, Bone Extract，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC50，測定時間：4.5 hours，測定因子：BMP-4 別名：BMP-2B Genbank No： 652 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年11月15日号 p.19
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| 製品記事 | R&D Systems 社 BMP-4関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Bone Extracts (50 µL)
- Sensitivity
- 3.68 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts)
- Specificity
- Natural and recombinant human BMP-4
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Bone Extracts
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 301 | 719 | 1,351 | 289 | 734 | 1,429 |
| Standard Deviation | 15.9 | 22.5 | 60.1 | 16.9 | 42.7 | 75.4 |
| CV% | 5.3 | 3.1 | 4.4 | 5.8 | 5.8 | 5.3 |
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Recovery
The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=8) | 100 | 91-109 |
| 2 M Urea (n=2) | 97 | 91-105 |
| 2 M Guanidine-HCI (n=2) | 96 | 93-103 |
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Linearity
The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.
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Additional Infomation
- Molecule Information
- BMP-4
- Aliases
- undefined
- Entrez Gene IDs
- 652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
- Background
- BMPs (Bone Morphogenetic Proteins)
- BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
Baiocchi M, Biffoni M, Castelli G, Fatica A, Ferracin M, Negrini M, Peschle C, Sorrentino A, Tomaselli G, Valtieri M
Exp. Hematol. 2008 36:1035-46
Species: Human
Sample Type: Cell Culture Supernates
The performance of poly-epsilon-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4.
Ambrosio L, Baldini N, Battista S, Capitani O, Causa F, Esposito MT, Greco M, Guarino V, Lombardo B, Netti PA, Pastore L, Pinna S, Savarino L, Valentini S, Zeppetelli S
Biomaterials 2007 28:3101-9
Species: Human
Sample Type: Cell Culture Supernates
Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals.
Barry DA, Cooper GM, Corsi KA, Gharaibeh B, Huard J, Pollett JB, Weiss KR
Stem Cells 2007 25:2302-11
Species: Human
Sample Type: Cell Culture Supernates
Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes.
Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK
Dig. Dis. Sci. 2007 52:2766-74
Species: Human
Sample Type: Cell Culture Supernates
Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
Green J, Herrmann S, Scharfenberger A, Weber T
Bone 2006 39:1156-63
Species: Human
Sample Type: Tissue Homogenates
Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
Blum B, Haggard W, Miller L, Moseley J, Richelsoph K
Orthopedics 2004 27:161-5
Species: Rat
Sample Type: Tissue Homogenates
Oncogenic beta-catenin is required for bone morphogenetic protein 4 expression in human cancer cells.
Crooks H, Dracheva T, Jen J, Kim JS, Nishanian TG, Singh B, Waldman T
Cancer Res. 2002 62:2744-8
Species: Human
Sample Type: Cell Culture Supernates
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