Human IL-18 BPa Quantikine ELISA Kit | hIL-18 BPa QKit
掲載日情報：2016/08/18 現在Webページ番号：111029
R&D Systems製のHuman IL-18 BPaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Human IL-18 BPa Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Human IL-18 BPa Quantikine ELISA Kit
文献数： 7
- 商品コード：DBP180
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：7.52 pg/mL，測定範囲：26.6-1,700 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Human，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC27，測定時間：4.5 hours，測定因子：IL-18 BPa 別名：IL18 BPa Genbank No： 10068 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Quantikine Human IL-18 BPa ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
- Sensitivity
- 7.52 pg/mL
- Assay Range
- 26.6 - 1,700 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IL-18 BPa
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtained using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 123 | 404 | 789 | 136 | 434 | 841 |
| Standard Deviation | 5.5 | 9.49 | 21.1 | 9.47 | 32.8 | 59.1 |
| CV% | 4.5 | 2.3 | 2.7 | 7 | 7.6 | 7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 109 | 368 | 740 | 115 | 362 | 692 |
| Standard Deviation | 5.73 | 16.4 | 15.8 | 13.5 | 29.1 | 48.3 |
| CV% | 5.3 | 4.5 | 2.1 | 11.7 | 8 | 7 |
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Recovery
The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 102 | 92-113 |
| EDTA Plasma (n=4) | 106 | 93-113 |
| Heparin Plasma (n=4) | 105 | 92-119 |
| Cell Culture Supernates (n=4) | 106 | 98-114 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- IL-18 BPa
- Aliases
- IL18BP
- Entrez Gene IDs
- 10068 (Human)
- Background
- IL-18 Binding Proteins
- Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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