Human BDNF Quantikine ELISA Kit | hBDNF QKit
掲載日情報：2016/08/18 現在Webページ番号：111026
R&D Systems製のHuman BDNFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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BDNF, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
BDNF, Human, ELISA Kit, Quantikine (96well)
文献数： 68
- 商品コード：DBD00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：カルタヘナ該当品
| 説明文 | 測定範囲：62.5-4,000 pg/mL，感度：20 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC22，測定時間：3.5 hours，測定因子：Free BDNF 別名：abrineurin Genbank No： 627 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | カルタヘナ該当品 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2019年11月15日号 p.14
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| 製品記事 | R&D Systems社 BDNF関連製品 ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor Plasma(EDTA, heparin, citrate) (50 uL)
- Sensitivity
- 20 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human BDNF
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 476 | 1,258 | 2,175 | 528 | 1,338 | 2,287 |
| Standard Deviation | 24 | 48.2 | 135.7 | 59.7 | 102.3 | 186 |
| CV% | 5 | 3.8 | 6.2 | 11.3 | 7.6 | 8.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 334 | 825 | 1,339 | 358 | 905 | 1,517 |
| Standard Deviation | 15.6 | 28.3 | 56.5 | 36.8 | 86.8 | 121.6 |
| CV% | 4.7 | 3.4 | 4.2 | 10.3 | 9.6 | 8.1 |
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Recovery
The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 100 | 85-116 |
| Platelet-poor Heparin Plasma (n=5) | 93 | 81-119 |
| Platelet-poor Citrate Plasma (n=5) | 99 | 87-120 |
| Platelet-poor EDTA Plasma (n=5) | 96 | 89-115 |
| Cell Culture Media (n=5) | 96 | 83-105 |
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Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- BDNF
- Aliases
- Abrineurin; ANON2; BULN2
- Entrez Gene IDs
- 627 (Human); 12064 (Mouse); 24225 (Rat)
- Background
- BDNF
- Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) thatare required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheralnervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. Thesesecreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. Allneurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all shareapproximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalentlylinked homodimer.BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yieldthe 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. Highlevels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels ofBDNF expression are found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinityand specifically activates the TrkB tyrosine kinase receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Bortezomib-induced peripheral neuropathy is related to altered levels of brain-derived neurotrophic factor in the peripheral blood of patients with multiple myeloma.
Akria , Luiza, Azoulay , David, Ben-Yehuda , Dina, Braester , Andrei, Gatt , Moshe E, Horowitz , Netanel, Lavie , David, Perlman , Riki, Suriu , Celia
Br J Haematol 2014 164:454-6
Species: Human
Sample Type: Serum
Exercise training increases size of hippocampus and improves memory.
Erickson KI, Prakash RS, Voss MW
Proc. Natl. Acad. Sci. U.S.A. 2011 108:3017-22
Species: Human
Sample Type: Serum
CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
Agulla J, Arias S, Brea D, Castillo J, Perez-Mato M, Rodriguez-Yanez M, Sobrino T
J. Neurosci. Res. 2011 89:979-85
Species: Human
Sample Type: Serum
Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
Gehring M, Kapp A, Mommert S, Novak N, Pfab F, Raap U, Rossing K, Wedi B
Clin. Exp. Allergy 2011 41:1392-1399
Species: Human
Sample Type: Serum
Androgen regulates brain-derived neurotrophic factor in spinal motoneurons and their target musculature.
Cai Y, Osborne MC, Sengelaub DR, Verhovshek T
Endocrinology 2010 151:253-61
Species: Rat
Sample Type: Tissue Homogenates
Endurance training enhances BDNF release from the human brain.
Adser H, Brassard P, Jakobsen AH, Nielsen HB, Nordby P, Pilegaard H, Rasmussen P, Secher NH, Seifert T, Stallknecht B, Wissenberg M
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2010 298:R372-7
Species: Human
Sample Type: Plasma
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Cheng CC, Chiu TH, Hsu SH, Huang YT, Lai PC, Lan YF, Wu CC
Int. J. Oncol. 2010 36:1469-76
Species: Human
Sample Type: Cell Culture Supernates
Spinal adenosine A2a receptor activation elicits long-lasting phrenic motor facilitation.
Baker-Herman TL, Golder FJ, Hoffman M, Lovett-Barr MR, Mitchell GS, Ranganathan L, Satriotomo I, Watters JJ
J. Neurosci. 2008 28:2033-42
Species: Rat
Sample Type: Tissue Homogenates
Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
Amin MB, Chung LW, Coleman IM, Dahut WL, Figg WD, Hsieh CL, Johnstone PA, Law A, Lee JK, Lim S, Lyles R, Marshall FF, Multani AS, Nelson P, Pathak S, Sung SY, Wu LC, Zhau HE
Cancer Res. 2008 68:9996-10003
Species: Human
Sample Type: Serum
Decreased brain-derived neurotrophic factor (BDNF)- and beta-thromboglobulin (beta-TG)- blood levels in Alzheimer's disease.
Eschweiler GW, Gawaz M, Langer H, Laske C, Leyhe T, Schott K, Stransky E
Thromb. Haemost. 2006 96:102-3
Species: Human
Sample Type: Serum
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