Human PDGF-BB Quantikine ELISA Kit | hPDGF-BB QKit
掲載日情報：2016/08/18 現在Webページ番号：111024
R&D Systems製のHuman PDGF-BBを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月28日 08時15分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
PDGF-BB, Human, ELISA Kit, Quantikine (96well) |
|
66 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年05月28日 08時15分現在]
PDGF-BB, Human, ELISA Kit, Quantikine (96well)
文献数： 66
- 商品コード：DBB00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：15 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC22，測定時間：4.5 hours，測定因子：PDGF-BB 別名：PDGF-B |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
||||||
| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
||||||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
- Sensitivity
- 15 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human PDGF-BB
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 310 | 759 | 1,093 | 287 | 772 | 1,082 |
| Standard Deviation | 14 | 18.6 | 25.5 | 21.9 | 56.9 | 89.8 |
| CV% | 4.5 | 2.4 | 2.3 | 7.6 | 7.4 | 8.3 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 268 | 639 | 935 | 251 | 655 | 918 |
| Standard Deviation | 11.3 | 28.4 | 26.8 | 22.4 | 38.5 | 59.3 |
| CV% | 4.2 | 4.4 | 2.9 | 8.9 | 5.9 | 6.5 |
追加しました。
Recovery
The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 102 | 93-114 |
| Platelet-poor EDTA Plasma (n=6) | 101 | 88-115 |
| Platelet-poor Heparin Plasma (n=6) | 103 | 89-112 |
| Cell Culture Media (n=4) | 94 | 86-102 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- PDGF-BB
- Aliases
- undefined
- Entrez Gene IDs
- 5155 (Human); 24628 (Rat)
- Background
- PDGF-A, -B, and -D
- The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Bieback K, Dietz L, Kinzebach S, Kluter H, Thierse H
BMC Cell Biol 2013 14:48
Species: Human
Sample Type: Cell Lysates
Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.
Fujii T, Kuwano H, Onimaru M, Sueishi K, Yonemitsu Y
Am. J. Physiol. Heart Circ. Physiol. 2008 294:H2785-91
Species: Human
Sample Type: Cell Culture Supernates
Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
Baumgart-Vogt E, Dingeldein E, Franke RP, Fuhrmann R, Hild A, Jonuleit T, Karnati S, Kilian O, Schnettler R, Wenisch S
Biomaterials 2008 29:3429-37
Species: Porcine
Sample Type: Plasma
Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
Arimura A, Hikita I, Kawabata T, Matsushima S, Nagira M, Oku H, Shimizu T, Torii M, Ueyama A
Eur. J. Pharmacol. 2008 590:400-8
Species: Mouse
Sample Type: Tissue Homogenates
Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
Bucana C, Fidler IJ, Horne E, Jones D, Lee YC, Lin SH, Logothetis CJ, Mathew P, Morris MJ, Oh WK, Thall PF, Wen S
Br. J. Cancer 2008 99:1426-32
Species: Human
Sample Type: Plasma
Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
Dermietzel R, Grunwald T, Jensen A, Meier C, Moers J, Neuhoff S, Rieks M
Exp. Hematol. 2007 35:1119-31
Species: Human
Sample Type: Cell Culture Supernates
TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
Aberg M, Johnell M, Nordin A, Siegbahn A, Velling T
Arterioscler. Thromb. Vasc. Biol. 2007 28:135-41
Species: Porcine
Sample Type: Cell Culture Supernates
Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Dong J, Ferrara N, Frantz GD, Gerber HP, Hall L, Jung K, Liang X, Meng G, Peale FV, Tejada ML, Yu L
Clin. Cancer Res. 2006 12:2676-88
Species: Human
Sample Type: Cell Culture Supernates
Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
Green J, Herrmann S, Scharfenberger A, Weber T
Bone 2006 39:1156-63
Species: Human
Sample Type: Plasma
Effects of simvastatin on angiogenic growth factors released at the site of microvascular injury.
Celinska-Lowenhoff M, Nizankowski R, Stepien E, Szczeklik A, Tracz W, Undas A
Thromb. Haemost. 2006 95:1045-7
Species: Human
Sample Type: Plasma
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。