Human Amphiregulin Quantikine Immunoassay | hAmphiregulin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111019
R&D Systems製のHuman Amphiregulin Quantikine Immunoassayを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月02日 12時35分現在]
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Amphiregulin, Human, ELISA Kit, Quantikine |
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[在庫・価格 ：2024年05月02日 12時35分現在]
Amphiregulin, Human, ELISA Kit, Quantikine
文献数： 10
- 商品コード：DAR00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：15.60 - 1000 pg/mL，感度：3.56 pg/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)，種交差性：Human，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC40，測定時間：4.5 hours，測定因子：Amphiregulin 別名：amphiregulin Genbank No： 374 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human Amphiregulin ELISA Kit Anti-Human ROR2 Allophycocyanin Monoclonal Antibody (Clone 231512) |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)
- Sensitivity
- 3.56 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Cell Lysates, EDTA Plasma, Urine)
- Specificity
- Recombinant and natural human Amphiregulin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Amphiregulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Amphiregulin in cell culture supernates, cell lysates serum, plasma, saliva, and urine. It contains E. coli-derived recombinant human Amphiregulin and has been shown to accurately quantitate the recombinant factor. Results obtained with natural human Amphiregulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amphiregulin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 102 | 314 | 631 | 99.4 | 308 | 614 |
| Standard Deviation | 5.63 | 10.5 | 22.4 | 9.85 | 20.5 | 28.8 |
| CV% | 5.5 | 3.3 | 3.5 | 9.9 | 6.7 | 4.7 |
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Recovery
The recovery of Amphiregulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 95 | 85-103 |
| Cell Culture Media (n=4) | 95 | 88-101 |
| EDTA Plasma (n=4) | 93 | 82-105 |
| Heparin Plasma (n=4) | 94 | 82-103 |
| Saliva (n=4) | 104 | 98-113 |
| Urine (n=4) | 88 | 75-99 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Amphiregulin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- Amphiregulin
- Aliases
- AREG
- Entrez Gene IDs
- 374 (Human); 11839 (Mouse)
- Background
- Amphiregulin (AR)
- Amphiregulin is a member of the EGF family of cytokines, which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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