Human alpha-Fetoprotein Quantikine ELISA Kit | hAFP QKit
掲載日情報：2016/08/18 現在Webページ番号：111010
R&D Systems製のHuman alpha-Fetoproteinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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AFP, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
AFP, Human, ELISA Kit, Quantikine (96 well)
文献数： 11
- 商品コード：DAFP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.312-20 ng/mL，感度：0.046 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC38，測定時間：4.5 hours，測定因子：alpha-Fetoprotein, AFP 別名：AFP Genbank No： 174 |
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| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2018年7月1日号 p.11
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| 製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit Mouse α-Fetoprotein Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.046 ng/mL
- Assay Range
- 0.312 - 20 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural human AFP
- Interference
- Interference observed with other substances.
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Product Summary
The Quantikine Human AFP immunoassay is a 4.5 hour solid phase ELISA designed to measure human AFP in cell culture supernates, serum, and plasma.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 2.89 | 6.02 | 12.3 | 2.65 | 5.62 | 11.7 |
| Standard Deviation | 0.109 | 0.219 | 0.441 | 0.158 | 0.261 | 0.549 |
| CV% | 3.8 | 3.6 | 3.6 | 6 | 4.6 | 4.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3.15 | 6.62 | 13.2 | 3.22 | 6.79 | 14.1 |
| Standard Deviation | 0.08 | 0.149 | 0.447 | 0.215 | 0.382 | 0.93 |
| CV% | 2.5 | 2.3 | 3.4 | 6.7 | 5.6 | 6.6 |
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Recovery
The recovery of AFP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 103 | 86-115 |
| Heparin Plasma (n=4) | 103 | 95-115 |
| EDTA Plasma (n=4) | 104 | 94-114 |
| Cell Culture Media (n=4) | 99 | 95-103 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of AFP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- alpha-Fetoprotein/AFP
- Aliases
- AFP; DSCAM2
- Entrez Gene IDs
- 174 (Human); 11576 (Mouse); 24177 (Rat)
- Background
- alpha-Fetoprotein
- alpha-Fetoprotein (AFP), a member of the albuminoid superfamily, is a fetal/tumor-associated protein that is a marker for certain cancers and congenital defects. Like other members of the albuminoid family, AFP can act as a carrier protein, binding several types of molecules including steroids, bilirubin, fatty acids, retinoids, and flavanoids. Some studies suggest that AFP may also act as a regulator of cell growth and survival.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Knockdown of beta-catenin with dicer-substrate siRNAs reduces liver tumor burden in vivo.
Arvan R, Basu S, Behlke M, Brown B, Collingwood M, Cyr W, Diwanji R, Dudek H, Farkiwala R, Holmes B, Lee M, Li Y, Rettig G, Shah A, Wong D, Wortham K, Yang H, Ying B, Zhou W, Zhou Y
Mol Ther 2014 22:92-101
Species: Human
Sample Type: Serum
Proteomics mapping of cord blood identifies haptoglobin "switch-on" pattern as biomarker of early-onset neonatal sepsis in preterm newborns.
Abdel-Razeq SS, Bhandari V, Bizzarro M, Buhimschi CS, Buhimschi IA, Dulay AT, Han YW, Nayeri UA, Pettker CM, Thung S, Zhao G
PLoS ONE 2011 6:e26111
Species: Human
Sample Type: Serum
DLK1, a serum marker for hepatoblastoma in young infants.
Aronson DC, Falix FA, Hiralall JK, Lamers WH, Seppen J
Pediatr Blood Cancer 2011 59:743
Species: Human
Sample Type: Serum
A hypoxia- and alpha-fetoprotein-dependent oncolytic adenovirus exhibits specific killing of hepatocellular carcinomas.
Huyn S, Kim M, Kim PH, Kwon OJ, Wu L, Yun CO
Clin. Cancer Res. 2010 16:6071-82
Species: Mouse
Sample Type: Serum
Identifying serological biomarkers of hepatocellular carcinoma using surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy.
Ban KC, Huang S, Liu JY, Wang Q, Wu FX, Yuan WP, Zhang ZM, Zhao YN
Cancer Lett. 2009 279:163-70
Species: Human
Sample Type: Serum
A novel approach and protocol for discovering extremely low-abundance proteins in serum.
Akiyama H, Hirano H, Jung G, Kawasaki H, Kuroda T, Sugaya H, Tanahashi K, Tanaka Y, Utsumi J
Proteomics 2006 6:4845-55
Species: Human
Sample Type: Serum
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