Human IL-34 Quantikine ELISA Kit | hIL-34 QKit
掲載日情報：2016/08/18 現在Webページ番号：111000
R&D Systems製のHuman IL-34を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月25日 20時15分現在]
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Human IL-34 Quantikine ELISA Kit |
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[在庫・価格 ：2024年04月25日 20時15分現在]
Human IL-34 Quantikine ELISA Kit
文献数： 4
- 商品コード：D3400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：3.06 pg/mL，測定範囲：31.30 - 2000 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Human Milk (13 uL)，種交差性：Human，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC31，測定時間：4.5 hours，測定因子：IL-34 Genbank No： 146433 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
R＆D Systems社のIL-34関連製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Human Milk (13 µL)
- Sensitivity
- 3.06 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-34
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IL-34 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-34 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-34. Results obtained using natural human IL-34 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-34.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 212 | 571 | 1,174 | 215 | 597 | 1,206 |
| Standard Deviation | 15.5 | 14.4 | 21.2 | 12.8 | 35.4 | 50 |
| CV% | 7.3 | 2.5 | 1.8 | 6 | 5.9 | 4.1 |
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Recovery
The recovery of human IL-34 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 97 | 85-104 |
| Serum (n=4) | 98 | 90-106 |
| EDTA Plasma (n=4) | 97 | 90-102 |
| Heparin Plasma (n=4) | 97 | 88-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-34 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- IL-34
- Aliases
- C16orf77
- Entrez Gene IDs
- 146433 (Human); 76527 (Mouse)
- Background
- IL-34
- Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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