Canine CCL2/MCP-1 Quantikine ELISA Kit | caCCL2/MCP-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：110983
R&D Systems製のCanine CCL2/MCP-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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MCP-1, Canine, ELISA Kit, Quantikine (96well) <CCL2 ELISA Kit> |
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[在庫・価格 ：2025年04月26日 20時55分現在]
MCP-1, Canine, ELISA Kit, Quantikine (96well) <CCL2 ELISA Kit>
文献数： 4
- 商品コード：CACP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：15.6-1,000 pg/mL，感度：10.8 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Canine，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：CCL2, JE, MCP-1 別名：C-C motif chemokine ligand 2 Genbank No： 6347 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | CCL2/MCP-1 Quantikine/DuoSet ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 10.8 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant canine MCP-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Canine MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine MCP-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine MCP-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine MCP-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 43 | 43 | 43 |
| Mean | 37.1 | 144 | 632 | 42.7 | 133 | 655 |
| Standard Deviation | 2.2 | 5.8 | 22 | 5.4 | 7.5 | 30 |
| CV% | 5.9 | 4 | 3.5 | 12.6 | 5.6 | 4.6 |
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Recovery
The recovery of canine MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 93 | 84-102 |
| Heparin Plasma (n=4) | 103 | 96-112 |
| Cell Culture Supernates (n=4) | 112 | 106-121 |
| EDTA Plasma (n=4) | 110 | 102-116 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of canine MCP-1 in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- CCL2/JE/MCP-1
- Aliases
- MCAF; MCP-1
- Entrez Gene IDs
- 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
- Background
- CCL2/MCP-1/JE
- MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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