Canine IL-6 Quantikine ELISA Kit | caIL-6 Qkit
掲載日情報：2016/08/18 現在Webページ番号：110981
R&D Systems製のCanine IL-6を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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IL-6, Canine, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
IL-6, Canine, ELISA Kit, Quantikine (96 well)
文献数： 9
- 商品コード：CA6000
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：31.30 - 2000 pg/mL，感度：11.8 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)，種交差性：Canine，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-6 別名：B cell stimulatory factor-2 Genbank No： 3569 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 11.8 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant canine IL-6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Canine IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural canine IL-6 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring canine IL-6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 67 | 65 | 66 |
| Mean | 92.6 | 256 | 685 | 90.7 | 262 | 666 |
| Standard Deviation | 3.1 | 8.3 | 15.3 | 6.6 | 15.5 | 30.2 |
| CV% | 3.3 | 3.2 | 2.2 | 7.3 | 5.9 | 4.5 |
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Recovery
The recovery of canine IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 93 | 84-102 |
| Heparin Plasma (n=4) | 92 | 83-100 |
| Cell Culture Supernates (n=6) | 94 | 87-103 |
| EDTA Plasma (n=4) | 97 | 91-103 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with various concentrations of canine IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial.
Candolfi M, Liu C, Xiong W
PLoS ONE 2010 5:e11074
Species: Canine
Sample Type: Cell Culture Supernates
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