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Can be used to synthesize membrane proteins NanoDisc BD Kit

Date：April 15 2023Web Page No：95035

NanoDisc BD Kit is a set of reagents necessary for NanoDisc synthesis for membrane protein with good quality and yield.
* This product is only available in US.

Membrane protein synthesis by wheat germ cell free protein expression system
Features
Recommended for
Procedure
Product information

Membrane protein synthesis by wheat germ cell free protein expression system

Although nearly one-third of all eukaryotic genes encode membrane proteins, there are still many challenges in their functional and structural analysis. This is mainly due to the fact that membrane proteins can be toxic to cellular systems or form insoluble aggregates, and the complexity of expressing them in standard protein expression systems. CellFree Sciences Co., Ltd. has previously synthesized membrane proteins in the presence of various detergents and synthesized them as proteomic micelles and providing kits for synthesizing membrane proteins as proteoliposomes.

And now they newly released a Kit for membrane protein synthesis by wheat germ cell free protein synthesis system,“NanoDisc BD Kit”.

NanoDisc, prepared by various methods, have already been reported for membrane protein expression and research. This kit takes advantage of the benefits of the wheat cell-free synthesis system and combines WEPRO7240ND, a wheat germ extract optimized for Nanodisc synthesis, with the BD method, which has a proven track record in proteoliposome synthesis, and a new synthesis method (patent pending) that co-expresses mRNA for MSP protein and mRNA for the target membrane protein. This new synthesis method (patent pending) enables the synthesis of nanodiscs with unprecedented efficiency and higher quality. This kit enables high-quality and high-efficiency synthesis of membrane proteins that have been difficult to express in the past and is expected to further advance research on functional and structural analysis using membrane proteins.

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Features

NanoDisc has been synthesized using co-expression with Asolectine liposome, mRNA for MSP ( Membrane Scaffold Protein) synthesis and mRNA for target membrane protein.
Can be used for 5 reactions on 2ml of bilayer–dialysis method which has been established for proteoliposome sysnthesis.
Target proteins can be confirmed through SDS-PAGE/CBB staining.

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Recommended for

Researchers who require yields of membrane proteins several hundred micrograms
Researchers who want to perform interaction analysis
Researchers who want to perform structure analysis of membrane protein

* This kit does not include dialysis device. A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.

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Procedure

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Product information

Product Name	Product Code	Supplier	Size	Storage
NanoDisc BD Kit	CFS-CNDE-BD	CFR	1 kit	-80°C

* This product is only available in US.

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CONTACT

export@funakoshi.co.jp

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