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Super-Photostable Near Infrared Dye PREX710-NHS

Date:December 28 2020Web Page No:95010

Funakoshi Co.,Ltd.

Super-Photostable Near Infrared Dye PREX710-NHS

PREX710 is a super-photostable near infrared (NIR) fluorescent dye with high chemical stability. PREX710 is more suitable for biological imaging, especially in vivo and deep imaging, than conventional cyanine dyes such as indocyanine green (ICG). Our format is PREX710-NHS (N-hydroxysuccinimide) which enables to label amino group-containing molecules such as proteins, antibodies, peptides, amino-nucleotides and any other small compounds.

*For US customers: this product is not available in US.
Background

Conventional NIR dyes are not enough for bio-imaging
Near infrared (NIR) dyes absorb and emit light in 700-900 nm, the out of visible light range. As the range of NIR shows low auto-fluorescence from biological samples, NIR dyes are one of the most suitable fluorophore for biological imaging, especially in vivo imaging. A lot of NIR dyes were previously developed and some dyes including indocyanine green (ICG) are commercially available. However almost NIR dyes including indocyanine green (ICG) are based on the cyanine-type dye which contains hydrophobic long polymethine structures and show poor chemical and photostability. Generally, cyanine-type NIR dyes are tend to decompose in the water rapidly and considered as not enough dyes for bio-imaging.

PREX710 enables us to perform long-term bioimaging
A novel NIR dye, PREX710 (Photo Resistant Xanthene Dye 710 nm) is different from conventional cyanine-based NIR dyes because its backbone is a xanthene containing phosphine oxide.

-PREX710 could be excited by around 640-750 nm (max ~710 nm) and emit around 700-800 nm (max ~760 nm).
-PREX710 shows very highly chemical stability in both water and serum and also exhibits strong photostability.

Combining the above two advantages of PREX710 enables us to monitor in vivo imaging for long term, single molecule imaging, multiplex imaging with visible light fluorophores. PREX710 has been validated in in vivo imaging such as a deep blood vessel imaging in mouse brain, plant imaging omitting auto-fluorescence of chloroplasts, and stable single molecule imaging.

Supplied format: PREX710-NHS
PREX710-NHS (N-hydroxysuccinimide) ester is a reactive form of PREX710 dye suitable for the labeling of amino group containing molecules such as proteins, antibodies, peptides, amino-nucleotides and any other small compounds. PREX710 conjugates can be applied in various NIR imaging with highly chemical stability and super-photostability.


Features


  • Higher chemical stability compared with conventional dyes.
    High pH stability (pH 4 to 10) / High stability in serum and water / High photostability

   Photostability of PREX710-conjugated antibody under Xe lamp irradiation
   Normalized absorbance of PREX710-, competitive NIR dye "A"- and "C" -conjugated antibodies under irradiation with high power (300 W) Xe lamp    was monitored at each maximum wavelength.
Higher chemical stability compared with conventional dyes.

  • Low auto-fluorescence from biological samples.
    When applied in plant cells, there is extremely low auto-fluorescence from chloroplasts.
  • Label amino group containing molecules by conjugated NHS ester
  • Ex./Em. = 710 nm/ 740 nm
  • Various applications
  • Quantum yield: 0.12

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Applications

  • in vivo imaging / deep imaging
  • Plant imaging omitting autofluorescence of chloroplasts
  • Multi color imaging with visible light fluorophore

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Application data

In vivo deep imaging of blood vessels in live mouse brain
PREX710-dextran conjugate was injected into bloodstream via a tail vein of young adult ICR mouse. The brain tissue was monitored by “openslull” method with confocal microscopy (Ex./Em. = 638 nm/ 667-733 nm).
PREX710 was highly stable in blood and could be excited by near-infrared light which has little effect on hemoglobin.
in-vivo-deep imaging of blood vessels in live mouse brain


Single molecule imaging
Avidin protein was labeled with PREX710-NHS or cyanine-type NIR dye "A" and subsequently immobilized on a glass slide via biotin conjugated linker. Single molecule images of PREX710 and NIR dye "A" were acquired continuously at 10 frames per second for 120 sec with excitation light at 640 nm (60 W/cm2) in PBS (pH 7.4).
PREX710 showed high photostability under the single molecule level compared with NIR dye “ A ””. PREX710 has a great potential to apply into single molecular imaging.
single-cell-imaging

Plant imaging
Physcomitrella patens was stained with PREX710-conjugated octaarginine (R8) and fluorescent signal was observed by fluorescent microscopy. PREX710 (Ex./Em. = 703-717 nm/ 754-816 nm), chloroplast (Ex./Em. = 300-400 nm/ >420 nm).
As fluorescent signal of PREX710 (cyan) was clearly separated from chloroplast’s autofluorescence (red), PREX710 is a good fluorescent dye for plant imaging.
plant-imaging


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Reference data

Fluorescent spectrum
UV-Vis and normalized fluorescence spectra of free-PREX710 dye in 10 mM PBS buffer (pH 7.4). Blue; Absorption spectra. Red; Excitation spectra.
Fluorescent spectrum


Effect of pH on the stability of PREX710 dye
Normalized absorbance of free-PREX710 dye measured at maximum 712 nm for 10 hours using three different pH buffers in vitro. PREX710 is highly stable from pH 4 to 10.
Effect of pH on the stability of PREX710 dye

Chemical stability of PREX710 in blood serum
Free-PREX710 in PBS buffer (pH 7.4) were irradiated with high power (300 W) Xe lamp using continuous range of visible light 385-740 nm. The change of absorption spectra over time was monitored.
Chemical stability of PREX710 in blood serum

Photostability of PREX710 dye under Xe lamp irradiation
Normalized absorbance of free-PREX710 dye in 100% fetal bovine serum (FBS) was monit ored at maximum 712 nm in 10 min intervals for 10 hours in vitro.
Photostability of PREX710 dye under Xe lamp irradiation

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Chemical structure

chemical-structure
Formulation: C40H43ClN3O7P as chloride salt

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Reference

Grzybowski M et al., Angew. Chem. Int. Ed., 57, 10137~10141 (2018) A Highly Photostable Near-Infrared Labeling Agent Based on a Phospha-rhodamine for Long-Term and Deep Imaging.



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Price

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price
PREX710-NHS, Super-Photostable Dye
DatasheetThis may not be the latest data sheet.
FDV-0036 FNAFunakoshi Co.,Ltd. 1 mg $450

Description *This product is not available in US. PREX710 is a super-photostable near infrared (NIR) fluorescent dye with high chemical stability.
Storage -20°C CAS
Link

[Date : February 15 2024 12:37]

PREX710-NHS, Super-Photostable Dye


  • Product Code: FDV-0036
  • Supplier: FNA
  • Size: 1mg
  • Price: $450

Description *This product is not available in US. PREX710 is a super-photostable near infrared (NIR) fluorescent dye with high chemical stability.
Storage -20°C CAS
Link

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CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.