Serum-Free Media for Controlling the Proliferation Velocity of Human Fibroblasts Xyltech™ Growth H-Fbro / H-Fbro-01
Date：November 29 2020Web Page No：95005
These are two types of serum-free media designed for human fibroblasts. By combining Xyltech™ H-Fbro-01, which enables cultivation while suppressing cell growth, with Xyltech™ Growth H-Fbro for expansion and maintenance, users can precisely control the proliferation velocity of human fibroblasts.
＊The products are for research use only.
＊ Xyltech is a trademark of BBARL, Inc.
- About Fibroblasts
- Xyltech™ H-Fbro-01 (For Growth Regulation)
- Xyltech™ Growth H-Fbro (For Proliferation)
- Application Examples
- Precautions for Use
- Product Information
About Fibroblasts
Fibroblasts reside in the dermis layer of skin tissue and produce essential components such as collagen and hyaluronic acid. Human fibroblasts play a critical role in maintaining skin elasticity and healing damaged areas to preserve skin functions. In recent years, research and regenerative medicine applications using patient-derived, cultured fibroblasts have been actively advancing.
Xyltech™ H-Fbro-01 (For Growth Regulation)
Xyltech™ Growth H-Fbro (For Proliferation)
Application Examples
Time-lapse Comparison (72 hours)
Human fibroblasts cultured in Xyltech™ H-Fbro-01 show significantly lower proliferation rates compared to those cultured in Xyltech™ Growth H-Fbro.
Immunocytochemical Analysis of Human Fibroblasts (3 days of culture)
Top: Phase-contrast images
Bottom: Anti-Collagen I antibody staining (Alexa Fluor 568)
Results demonstrate that cells cultured with these media remain positive for Collagen I, confirming the maintenance of cellular characteristics.
Precautions for Use
Xyltech™ H-Fbro-01 and Growth H-Fbro do not contain substances that neutralize trypsin activity. When subculturing cells with trypsin as a cell dissociation reagent, it is strongly recommended that the trypsin activity be sufficiently neutralized with a trypsin inhibitor. Dilution washing alone does not completely remove trypsin activity. The remaining protease activity will reduce subsequent cell growth.
Product Information
[Date : August 11 2026 00:09]
Detail
Product Name
Product Code
Supplier
Size
Price
Xyltech H-Fbro-01 (#10301)
DatasheetThis may not be the latest data sheet.
87-281
KSKCell Science & Technology Institute, Inc.
100
ml
$128
Description
A serum-free culture medium that allows suppression of growth while culturing human fibroblasts. This product is a synthetic culture medium suitable for suppressive growth control of normal human fibroblasts. Xyltech H-Fbro-01 can be used to control the growth rate of human fibroblasts in combination with Xyltech Growth H-Fbro, which is a serum-free medium for human fibroblast proliferation. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture.
Storage
4°C,Dark Storage
CAS
Link
Xyltech Growth H-Fbro (#10311)
DatasheetThis may not be the latest data sheet.
87-282
KSKCell Science & Technology Institute, Inc.
500
ml
$162
Description
A serum-free culture medium for maintenance culture of human fibroblasts.This product is a synthetic culture medium suitable for cell growth of normal human fibroblasts. When used in combination with Xyltech H-Fbro-01, human fibroblast proliferation can be controlled as needed. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture.
Storage
4°C,Dark Storage
CAS
Link
[Date : August 11 2026 00:09]
Xyltech H-Fbro-01 (#10301)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-281
- Supplier: KSK
- Size: 100ml
-
Price:
$128
Description
A serum-free culture medium that allows suppression of growth while culturing human fibroblasts. This product is a synthetic culture medium suitable for suppressive growth control of normal human fibroblasts. Xyltech H-Fbro-01 can be used to control the growth rate of human fibroblasts in combination with Xyltech Growth H-Fbro, which is a serum-free medium for human fibroblast proliferation. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture.
Storage
4°C,Dark Storage
CAS
Link
Xyltech Growth H-Fbro (#10311)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-282
- Supplier: KSK
- Size: 500ml
-
Price:
$162
Description
A serum-free culture medium for maintenance culture of human fibroblasts.This product is a synthetic culture medium suitable for cell growth of normal human fibroblasts. When used in combination with Xyltech H-Fbro-01, human fibroblast proliferation can be controlled as needed. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture.
Storage
4°C,Dark Storage
CAS
Link
Related Product
[Date : August 11 2026 00:09]
Detail
Product Name
Product Code
Supplier
Size
Price
Artificial Serum (Animal-free)(#A2G20P2CC)
87-082
KSKCell Science & Technology Institute, Inc.
20
ml
$1,049
Description
Artificial Serum (Animal-free) can be used as a replacement of FBS for serum-free cell culture medium. This product has been developed to obtain similar results of using FBS or human serum for cell culture.
Storage
-20°C,Dark Storage
CAS
Link
[Date : August 11 2026 00:09]
Artificial Serum (Animal-free)(#A2G20P2CC)
- Product Code: 87-082
- Supplier: KSK
- Size: 20ml
-
Price:
$1,049
Description
Artificial Serum (Animal-free) can be used as a replacement of FBS for serum-free cell culture medium. This product has been developed to obtain similar results of using FBS or human serum for cell culture.
Storage
-20°C,Dark Storage
CAS
Link
CONTACT
export@funakoshi.co.jp
- ※Prices on our website are for your reference only. Please inquire your distributor for your prices.
- ※Please note that Product Information or Price may change without notice.
Human fibroblasts cultured in Xyltech™ H-Fbro-01 show significantly lower proliferation rates compared to those cultured in Xyltech™ Growth H-Fbro.
Immunocytochemical Analysis of Human Fibroblasts (3 days of culture)
Top: Phase-contrast images
Bottom: Anti-Collagen I antibody staining (Alexa Fluor 568)
Results demonstrate that cells cultured with these media remain positive for Collagen I, confirming the maintenance of cellular characteristics.
Precautions for Use
Xyltech™ H-Fbro-01 and Growth H-Fbro do not contain substances that neutralize trypsin activity. When subculturing cells with trypsin as a cell dissociation reagent, it is strongly recommended that the trypsin activity be sufficiently neutralized with a trypsin inhibitor. Dilution washing alone does not completely remove trypsin activity. The remaining protease activity will reduce subsequent cell growth.
Product Information
[Date : August 11 2026 00:09]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Xyltech H-Fbro-01 (#10301) DatasheetThis may not be the latest data sheet. |
87-281 | KSKCell Science & Technology Institute, Inc. | 100 ml | $128 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
|
Xyltech Growth H-Fbro (#10311) DatasheetThis may not be the latest data sheet. |
87-282 | KSKCell Science & Technology Institute, Inc. | 500 ml | $162 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
[Date : August 11 2026 00:09]
Xyltech H-Fbro-01 (#10301)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-281
- Supplier: KSK
- Size: 100ml
- Price: $128
| Description |
A serum-free culture medium that allows suppression of growth while culturing human fibroblasts. This product is a synthetic culture medium suitable for suppressive growth control of normal human fibroblasts. Xyltech H-Fbro-01 can be used to control the growth rate of human fibroblasts in combination with Xyltech Growth H-Fbro, which is a serum-free medium for human fibroblast proliferation. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture. |
||
|---|---|---|---|
| Storage | 4°C,Dark Storage | CAS | |
| Link |
|
||
Xyltech Growth H-Fbro (#10311)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-282
- Supplier: KSK
- Size: 500ml
- Price: $162
| Description |
A serum-free culture medium for maintenance culture of human fibroblasts.This product is a synthetic culture medium suitable for cell growth of normal human fibroblasts. When used in combination with Xyltech H-Fbro-01, human fibroblast proliferation can be controlled as needed. This product is serum-free and contains no human or other animal-derived ingredients. If necessary, human serum (HS), fetal bovine serum (FBS), or serum substitute reagents can be added for cell culture. |
||
|---|---|---|---|
| Storage | 4°C,Dark Storage | CAS | |
| Link |
|
||
Related Product
[Date : August 11 2026 00:09]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Artificial Serum (Animal-free)(#A2G20P2CC) |
87-082 | KSKCell Science & Technology Institute, Inc. | 20 ml | $1,049 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
[Date : August 11 2026 00:09]
Artificial Serum (Animal-free)(#A2G20P2CC)
- Product Code: 87-082
- Supplier: KSK
- Size: 20ml
- Price: $1,049
| Description |
Artificial Serum (Animal-free) can be used as a replacement of FBS for serum-free cell culture medium. This product has been developed to obtain similar results of using FBS or human serum for cell culture. |
||
|---|---|---|---|
| Storage | -20°C,Dark Storage | CAS | |
| Link | |||
CONTACT
export@funakoshi.co.jp
- ※Prices on our website are for your reference only. Please inquire your distributor for your prices.
- ※Please note that Product Information or Price may change without notice.