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HOME> Products> Cell Culture> Medium> HFDM-1 medium

Serum-free medium, optimized for human fibroblast HFDM-1 medium

Date：November 29 2020Web Page No：95001

Cell Science & Technology Institute, Inc.

HFDM-1 medium is a fully synthetic culture medium for Normal Human Dermal Fibroblasts (NHDF), which enables serum-free culture while maintaining the same cell proliferation capacity as serum-containing culture medium. A small amount of human serum can also be added to promote cell proliferation.

Features
Composition
Example of Use
Product information

Features

Do not require the addition of cell adhesion factors, so it can be cultured on normal cell cultureware.
Completely synthetic medium based on RITC80-7 medium.
Proteins contained in this medium are all recombinant proteins.
No animal-derived ingredients (serum proteins, etc.).
Serum-free culture is possible, but the addition of a little (0.5-2.0%) human serum further enhances the cell proliferation effect (see Example of Use).
Osmotic pressure: 290-310 mOsm/kg H₂O
Filter sterilized.
Endotoxin tested.
pH: 7.2-7.6
Suitable for growth culture of normal human fibroblasts (HF).
Contains all the ingredients necessary to maintain the growth and function of normal human fibroblasts.

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Composition

HFDM-1 medium contains basic culture ingredients such as inorganic salts, amino acids, vitamins, nucleic acids, trace elements and carbohydrates, as well as cell growth factors such as recombinant human insulin and recombinant human epidermal growth factor (EGF) in a composition optimized for HF growth.

RITC80-7 culture medium
HEPES
L-glutamine
Lipid
Sodium bicarbonate
Sodium selenite
Recombinant human insulin
Recombinant human EGF
Synthetic polymer

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Example of Use

referencedata — Normal Human Dermal Fibroblasts (NHDF) in HFDM-1 medium

Left: NHDF cell growth
Serum-free HFDM-1 shows similar growth to serum-containing medium.

Right: Positive effect of human serum
Addition of human serum to HFDM-1 promotes NHDF proliferation.

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Product information

[Date : May 16 2025 00:07]

Detail Product Name Product Code Supplier Size Price

HFDM-1(+) Medium (#87-160) 2102P05 KSKCell Science & Technology Institute, Inc. 500 ml $88

Contact

Description	New code: 87-160
Storage	4°C,Dark Strage,Don't freeze	CAS
Link

HFDM-1(+) Medium (#87-162) 2102P10 KSKCell Science & Technology Institute, Inc. 1000 ml $110

Contact

Description	New code: 87-162
Storage	4°C,Dark Strage,Don't freeze	CAS
Link

[Date : May 16 2025 00:07]

HFDM-1(+) Medium (#87-160)

Product Code: 2102P05
Supplier: KSK
Size: 500ml
Price: $88

Description	New code: 87-160
Storage	4°C,Dark Strage,Don't freeze	CAS
Link

Contact

HFDM-1(+) Medium (#87-162)

Product Code: 2102P10
Supplier: KSK
Size: 1000ml
Price: $110

Description	New code: 87-162
Storage	4°C,Dark Strage,Don't freeze	CAS
Link

Contact

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