Myell™ series: Get clear pictures of Live Cell Imaging!

*Myell™ series are NOT sterilized.

When observing cells such as floating cells or yeast, dropping cell suspension on a glass bottom dish is general method. However, this method has a problem that the picture becomes blurry because the cells vibrate due to their brownian motion.
By wrapping with Myell™ A/S, the samples on a slide glass are prevented from vibrating. This makes us to get clear pictures.
Also, pictures hardly blurry even if some droplets are added on samples wrapped with Myell A/S.

Liposome suspention was wrapped or without wrapped by Myell™ A/S and applied to glass bottom dish.
Some drops were added on the both dishes and compared the pictures.

Without-wrapped

The liposome was flown out of the field in microscope.

Myell™ A/S-wrapped

The liposome remained in the field of microscope and did not have much vibrate.

Myell™ D prevents samples from not only vibrating but also the desiccation and shrink.
These features of Myell™ D is due to the material, CYTOP® which has both water-repellent and oil-repellent.
Then, Myell™ D is useful for long time imaging in a tiling mode and three dimensional mode.
Also, Myell™ D does not influence on image in fluorescence microscope.

The fixed brain section from GFP-expressed mouse was wrapped by Myell™ D or not.
Then after, the imaging was performed by a tiling mode as below.

The sample without-wrapped was desiccated and shrinked, consequently, the picture was blurry toward Z axis.
On the other hand, Myell™ D-wrapped sample was prevented from desiccating, which enables to get clear picture.

Conventionally, it is difficult to observe the effect of stimulation factor during live imaging because the samples are flown out once the stimulation factor is added.
Myell™ P has an advantage that adding stimulation factor on sample is possible while holding sample in microscopic field.
This is because Myell™ P has many small porous to penetrate.

The cells cannot penetrate this porous, however, the stimulation factor can penetrate.
Therefore, the cells remain inside and the effect of stimulation factor can be observed.
The blue ink was used as an example below.

*In case of using upright microscope
Please put sample which wrapped with Myell™ on slide glass, petri dish or directly on stage of microscope.
Upright microscope is suitable for Myell™ A/S and Myell™ D.

*In case of using inverted microscope
Please put sample which wrapped with Myell™ on petri dish.
This method is possible to soak water or embedded in agarose gel to prevent desiccation.
Inverted microscope is suitable for all Myell™ series.

*Note: For Myell™ A, one laboratory can order one time only.