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Say "goodbye" to gradient gel !! AllView PAGE Buffer

Date:November 05 2019Web Page No:81387

Say "goodbye" to gradient gel !!
AllView PAGE Buffer

AllView PAGE Buffer enable us to separate proteins widely during SDS-PAGE like gradient gel.

AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer has a remarkable feature that enable us to separate proteins with wide range of molecular weights in the basic Laemmli gel (Tris-HCl) similar to using “gradient gel”. The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. After electrophoresis, the polyacrylamide gel can be used directly for CBB staining, silver staining or western blotting.

Stacking gel3%
Resoling gel6%10%
BufferTris-Glycine-SDS bufferAllView PAGE BufferTris-Glycine-SDS bufferAllView PAGE Buffer
Gel Image6% SDS-PAGE gel10% SDS-PAGE gel

Widely separated! Only 13 min!
Sample: DynaMarker Protein MultiColor Stable II, Code #DM660
Fig. The mobility of proteins using AllView PAGE Buffer is different from Laemmli electrophoresis running buffer (Tris-Glycine-SDS). By using AllView PAGE Buffer and the Laemmli gels (Tris-HCl), a wide range of protein sizes can be separated like a gradient gels.

Feature

  • Save cost and time: gradient gel is not needed!
  • Only 13min to complete electrophoresis by mini gel.
  • Applicable to further assays: CBB staining, Western blot and etc...
  • 20x stock solution

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Protocol

  1. Dilute the AllView PAGE Buffer 20 times with ultrapure water. (e.g. Add 25 ml of AllView PAGE Buffer to 475 ml of ultrapure water.)
  2. Set the gel in electrophoresis chambers.
  3. Fill the chambers with the 1 × AllView PAGE Buffer.
  4. Load your samples and molecular weight markers.
  5. Start electrophoresis.

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Note

  • AllView PAGE Buffer is suitable for house-made Laemmli gels (see below “Recommended usage”).
  • If precast gels are used, the optimal acrylamide concentration may be different.
  • AllView PAGE Buffer is not reusable.
  • AllView PAGE Buffer is 20 × stock solution. Please dilute to 1 × solution before use.
  • If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
  • Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage.
  • Accordingly, it is recommended to monitor electrophoresis using a prestained MW Marker (e.g. DynaMarker® Protein MultiColor Stable II, Code#DM660).

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Movie

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Product Information

[Date : January 26 2021 23:29]

Detail Product Name Product Code Supplier Size Price
AllView PAGE Buffer
DatasheetThis may not be the latest data sheet.
DS520 BDLBioDynamics Laboratory Inc 500 ml $140

Description Say "goodbye" to gradient gel !! AllView PAGE Buffer enables to separate proteins widely during SDS-PAGE like gradient gel.
Storage RT CAS
Link

AllView PAGE Buffer/Protein MultiColor Stable II Set
DS522 BDLBioDynamics Laboratory Inc 1 set $35

Description
Storage 4°C CAS
Link

[Date : January 26 2021 23:29]

AllView PAGE Buffer


  • Product Code: DS520
  • Supplier: BDL
  • Size: 500ml
  • Price: $140

Description Say "goodbye" to gradient gel !! AllView PAGE Buffer enables to separate proteins widely during SDS-PAGE like gradient gel.
Storage RT CAS
Link

AllView PAGE Buffer/Protein MultiColor Stable II Set


  • Product Code: DS522
  • Supplier: BDL
  • Size: 1set
  • Price: $35

Description
Storage 4°C CAS
Link

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CONTACT

export@funakoshi.co.jp

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