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HOME> Products> Molecular Biology> Electrophoresis> AllView PAGE Buffer (20×)

Wide-Range Separation SDS-PAGE Running Buffer AllView PAGE Buffer (20×)

Date：November 05 2019Web Page No：81387

AllView PAGE Buffer (20×) is an innovative running buffer designed for protein SDS-PAGE. By simply replacing your conventional running buffer with this product, you can achieve wide-range protein separation across a broad molecular weight scale using standard, self-cast Laemmli gels—simulating the performance of a gradient gel.
Note: It is optimized for self-cast Laemmli gels and may not provide sufficient separation when used with precast gels.
＊The products are for research use only.

Video
Features
Testimonials
Technical Information: Quick Gel Preparation Recipe
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Product Information

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Electrophoresis conditions
Resolving gel percentage: 6%
Voltage (Constant): 250 V
Sample: ^DynaMarker Protein MultiColor Stable II (#DM660)
Running buffer: Conventional buffer (25 mM Tris・HCl, 192 mM Glycine, 0.1% SDS）
AllView PAGE Buffer (#DS520)

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Features

No Gradient Gel Required

This buffer eliminates the need to purchase precast gradient gels. Using a standard self-cast 6% polyacrylamide gel with AllView PAGE Buffer delivers a fractionation range equivalent to that of a gradient gel.
＊ See here for quick gel preparation recipe.

Comparison of Gradient Gel and AllView PAGE Buffer

Self-cast 6% polyacrylamide gel (5% C)^＊1 and ^DynaMarker Protein MultiColor Stable II (#DM660)^＊3 are utilized.

＊1 If using precast gels, the required gel concentration may differ or protein separation may be insufficient.
＊2 Conventional running buffer: Tris-Glycine SDS buffer
＊3 Apparent molecular weights may vary depending on the lot and running conditions. For details, see the datasheet attached to the product.

High Blotting Efficiency

Because it utilizes a low-concentration self-cast gel (6%), it offers higher western blot transfer efficiency compared to standard gradient gels. This ensures smoother and more complete protein transfer to PVDF membranes.

**Comparison of blotting efficiency between gels run with AllView PAGE Buffer and conventional gradient gels**
Self-cast gel (6%) ＋ AllView PAGE Buffer
Gradient gel ＋ Conventional running buffer^＊5

[Experimental Conditions]
Transfer method: Semi-dry blotting using a continuous buffer system (Towbin buffer containing 20% MeOH and SDS) and a PVDF membrane (0.2 μm)
Conditions: 2 mA/cm², 60 min
Sample: ^DynaMarker Protein MultiColor Stable II (#DM660)
＊5 Conventional electrophoresis buffer: Tris-Glycine SDS buffer

Wide-Range Separation on Any Gel Size

Wide Fractionation: Successfully resolves proteins ranging from 8 kDa to 230 kDa on a single gel concentration.
Midi-Gel Compatible: Works flawlessly with both mini-gels and large midi-gels (e.g., 140W × 140H mm)

High-Speed Electrophoresis

Time-Saving: Cuts total run time down to approximately 1/3 of traditional Tris-Glycine SDS buffers.
Run Times (at 250V constant):
・Mini-gels: ~15 minutes.
・Midi-gels: ~50 minutes.

Seamless Downstream Compatibility

Changing your buffer will not interrupt your existing workflow. After running with AllView PAGE Buffer, you can perform all standard downstream applications without modifications:

■ Silver Staining

Electrophoresis: 6% Polyacrylamide Gel / AllView PAGE Buffer

■ Western Blotting

WB with AllView PAGE Buffer — **Detection of Recombinant HSP70 Protein**

Electrophoresis: 6% Polyacrylamide Gel / AllView PAGE Buffer
Transfer Method: Semi-dry transfer using a continuous buffer system (Towbin buffer, 20% MeOH, SDS) and PVDF membrane (0.2 μm)
Primary Antibody: Anti-6×Histidine antibody
Secondary Antibody: HRP-conjugated anti-IgG antibody
Detection Reagent: ECL
Sample: Recombinant Mouse Hsp70

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Testimonials

Testimonial A (Research Institute):
"We perform western blots using large 20x20 cm self-cast gels. To detect both high and low molecular weight proteins, we used to run two separate gels (7% and 12%). With AllView PAGE Buffer, we can resolve everything beautifully on a single gel."

Testimonial B (University Laboratory):
"We wanted gradient-like separation without the high cost of commercial gradient precast gels. This buffer delivers exactly that using our own self-cast gels, making it incredibly convenient and budget-friendly."

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Technical Information: Quick Gel Preparation Recipe

By adding glycerol to the separating gel, you can cast both the separating and stacking layers simultaneously without waiting for the first layer to solidify.

① Separating gel solution (6% concentration, for 2 mini-gels)

Glycerol	1.5 mL
Ultrapure Water	6.55 mL
1.5M Tris-HCl (pH 8.8)	3.75 mL
30% Acrylamide / Bis solution	3.0 mL
10％ SDS	0.15 mL
TEMED	3.75 μL
10％ APS	0.05 mL

② Stacking gel solution (3% concentration, for 2 mini-gels)

Ultrapure Water	3.9 mL
0.5M Tris-HCl（pH6.8）	1.5 mL
30％ Acrylamide / Bis solution	0.6 mL
10％ SDS	0.06 mL
TEMED	3 μL
10％ APS	0.06 mL

③ Gel Polymerization

Pour the prepared separating gel solution (with glycerol) into the glass plate cassette.
Immediately and gently layer the stacking gel solution directly on top.
Allow it to sit at room temperature for approximately 1 hour to polymerize.
Tip: Setting it inside a 37°C incubator reduces polymerization time to about 30 minutes.

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Movie

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Product Information

[Date : July 31 2026 00:08]

Detail Product Name Product Code Supplier Size Price

AllView PAGE Buffer (20X)
DatasheetThis may not be the latest data sheet. DS520 BDLBioDynamics Laboratory Inc 500 ml $150

Contact

Description	AllView PAGE Buffer is a unique electrophoresis running buffer for SDS-PAGE. This running buffer enables a self-made gel to separate proteins with a wide range of molecular weights such as a gradient gel during SDS-PAGE.
Storage	RT	CAS
Link	Molecular Biology Product List

AllView PAGE Buffer (20X)/Protein MultiColor Stable II Set
DatasheetThis may not be the latest data sheet. DS522 BDLBioDynamics Laboratory Inc 1 set $40

Contact

Description	Limit One per Laboratory. Set of AllView PAGE Buffer (50 ml) and Protein MultiColor Stable II (100 μl).
Storage	4°C	CAS
Link

[Date : July 31 2026 00:08]

AllView PAGE Buffer (20X)

DatasheetThis may not be the latest data sheet.

Product Code: DS520
Supplier: BDL
Size: 500ml
Price: $150

Description	AllView PAGE Buffer is a unique electrophoresis running buffer for SDS-PAGE. This running buffer enables a self-made gel to separate proteins with a wide range of molecular weights such as a gradient gel during SDS-PAGE.
Storage	RT	CAS
Link	Molecular Biology Product List

Contact

AllView PAGE Buffer (20X)/Protein MultiColor Stable II Set

DatasheetThis may not be the latest data sheet.

Product Code: DS522
Supplier: BDL
Size: 1set
Price: $40

Description	Limit One per Laboratory. Set of AllView PAGE Buffer (50 ml) and Protein MultiColor Stable II (100 μl).
Storage	4°C	CAS
Link

Contact

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