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Running Buffer for SDS-PAGE with Wide Range Separation AllView PAGE Buffer (20×)

Date:November 05 2019Web Page No:81387

Electrophoresis running buffer for protein SDS-PAGE.
Simply replace your usual running buffer with this product. A self-made gel and AllView PAGE Buffer enable the separation (fractionation) of proteins with a wide range of molecular weights, similar to gradient gels.
Gels applied to this product are homemade gel by laemmli method.
*If you use a precast gel, the concentration of the applied gel may differ or the separation may be insufficient.

AllView Funako

Features

  • Gradient gel is not required
  • High blotting efficiency
  • Separates in a wide range
  • You can use a large gel!
  • High speed running: Just 15 minutes to complete electrophoresis by mini gel.
  • Applicable to further assays: CBB staining, Western blot and so on.

AllView PAGE Buffer enable us to separate proteins widely during SDS-PAGE like gradient gel.

Stacking gel 3%
Resoling gel 6%10%
Buffer Tris Glycine SDS buffer AllView PAGE Buffer Tris Glycine SDS buffer AllView PAGE Buffer
6% SDS-PAGE gel 10% SDS-PAGE gel

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Video

Electrophoresis conditions
Resolving gel percentage: 6%
Voltage (Constant): 250 V
Sample: DynaMarker Protein MultiColor Stable II (#DM660)
Running buffer: Conventional buffer (25 mM Tris・HCl, 192 mM Glycine, 0.1% SDS)
        AllView PAGE Buffer (#DS520)


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Testimonials

Customer reviews

Mr./Ms. A from a certain a research institute

■ Why I purchased AllView PAGE Buffer
I use my homemade gel (20 cm×20 cm) to do western every time. In order to see both high and low molecular weight molecules, I used 2 types of gel (12% and 7%) for electrophoresis, but I wanted to use only 1 type of gel, so I purchased this product.

■ What I am satisfied with
I am satisfied that I can detect a wide range of proteins from high to low molecular weight with one gel.


Mr./Ms. B from a university laboratory

■ Why I purchased AllView PAGE Buffer
I purchased it because I wanted to run and detect proteins similar to gradient gels.

■ What I am satisfied with
I can detect a wide range of proteins with this buffer and my own gel without purchasing gradient gels, which is very convenient.


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Note

  • Not reusable
  • 20 × stock solution. Please dilute to 1 × solution before use.
  • If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
  • Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage.

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Movie



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Product Information

[Date : July 15 2025 00:07]

Detail Product Name Product Code Supplier Size Price
AllView PAGE Buffer (20X)
DatasheetThis may not be the latest data sheet.
DS520 BDLBioDynamics Laboratory Inc 500 ml $150

Description AllView PAGE Buffer is a unique electrophoresis running buffer for SDS-PAGE. This running buffer enables a self-made gel to separate proteins with a wide range of molecular weights such as a gradient gel during SDS-PAGE.
Storage RT CAS
Link

Molecular Biology Product List

AllView PAGE Buffer (20X)/Protein MultiColor Stable II Set
DatasheetThis may not be the latest data sheet.
DS522 BDLBioDynamics Laboratory Inc 1 set $40

Description Limit One per Laboratory. Set of AllView PAGE Buffer (50 ml) and Protein MultiColor Stable II (100 μl).
Storage 4°C CAS
Link

[Date : July 15 2025 00:07]

AllView PAGE Buffer (20X)


  • Product Code: DS520
  • Supplier: BDL
  • Size: 500ml
  • Price: $150

Description AllView PAGE Buffer is a unique electrophoresis running buffer for SDS-PAGE. This running buffer enables a self-made gel to separate proteins with a wide range of molecular weights such as a gradient gel during SDS-PAGE.
Storage RT CAS
Link

Molecular Biology Product List

AllView PAGE Buffer (20X)/Protein MultiColor Stable II Set


  • Product Code: DS522
  • Supplier: BDL
  • Size: 1set
  • Price: $40

Description Limit One per Laboratory. Set of AllView PAGE Buffer (50 ml) and Protein MultiColor Stable II (100 μl).
Storage 4°C CAS
Link

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CONTACT

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