HOME> Products> Miscellaneous> Controllable NO Donor : NO-Rosa5

Controllable NO Donor : NO-Rosa5

Date:June 19 2019Web Page No:81329

Funakoshi Co.,Ltd.

NO donor, controlled by visible light
Controllable NO Donor : NO-Rosa5

Nitric oxide (NO), a gaseous free radical, is one of the signaling molecules produced by NO synthases physiologically. NO plays a key role in a wide range of biological processes.
However, NO is extremely unstable in physiological condition, so it is difficult to handle NO molecule in biological experiments.
This made all tough to analyze further function of NO in cells.
To overcome this point, "NO donors" which are compounds releasing NO molecules in the aqueous buffers have been used in NO research. Although NO donors are valuable tools, NO donors are not controllable spatio-temporally.

Controllable NO donor (original name; NO-Rosa5) is a novel NO donor controllable by visible light.
Controllable NOdonor has two moieties, rosamine fluorescent dye as light harvesting group and N-nitrosoaminophenol structure as NO releasing moiety and releases NO molecules triggered by photoinduced electron transfer (PeT).
It shows low photo-toxicity, low cytotoxicity, and efficient NO releasing by yellowish green light irradiation.

This product has been commercialized with the support of Nagoya City University.

Principle

Go back to index

Principle

  • Formulation : C34H33N4O5Cl
  • Molecular weight : 613.11 g/mol as a mono chloride salt
  • Solubility : Soluble in DMSO

Go back to index

Procedure

Go back to index

Selection guide of compatible fluorophores

Due to Controllable NOdonor releasing NO molecule upon 500-600 nm photo-irradiation, fluorophores, including fluorescent dyes, fluorescent reagent, and fluorescent proteins which are excitable by 500-600 nm wavelength are not available.
Furthermore, Controllable NOdonor itself shows red fluorescent emission. When customers use any fluorophore with Controllable NOdonor, please refer to following information.

(1) Fluorophores not recommended
- Excitation with 500-600 nm such as red fluorophores (for example Cy3, TAMRA, RFP protein etc.)

(2) Suitable fluorophores
- Excitation with <500 nm such as blue fluorophores (for example coumarin dye, BFP protein etc.) or green fluorophores (for example fluorescein, GFP protein etc.)
- Excitation with >600 nm such as near infrared red or far red fluorophores (for example Cy5, Cy7 etc.)

Go back to index

Application Data

Fig.1 In cellullo region-specific NO generation with Controllable NOdonor
HEK293T cells were firstly treated with DAF-FM DA (10 uM) for 30 min. After washing, the cells were subsequently treated with 10 uM Controllable NOdonor for 60 min.
The dish was photoirradiated inside the indicated white circle (r=31 um) using a 543 nm laser of confocal microscopy.
Left; before irradiation, Right; after irradiation.
Only inside the white circle, NO was detected. (scale bar = 40 um)

Fig.2 Visible light-induced NO releasing in vitro
Quantitative analysis of NO release from Controllable NOdonor with NO electrode. Controllable NOdonor (final 10uM) in 100 mM HEPES buffer was irradiated with a MAX-303 (Asahi Spectra)equipped with a 530-590 nm band-pass filter (light intensity: 70 mW/cm2) .
Left: Continuous irradiation. Right: Short pulse irradiation.

Go back to index

Reference

1. Ieda et al., Sci. Rep., 9, 1430 (2019)
Structure-efficiency relationship of photoinduced electron transfer-triggered nitric oxide releasers.
2. Ieda et al., Chem. Pharm. Bull., 67, 576-579 (2019) ,
In cellullo and ex vivo availability of yellowish-green-light-controllable NO releaser.
3. Okuno et al., Org. Biomol. Chem., 15, 2791-2796 (2017)
A yellowish-green-light-controllable nitric oxide donor based on N-nitrosoaminophenol applicable for photocontrolled vasodilation.

Go back to index

Product Information

[Date : December 10 2019 23:16]

Detail Product Name Product Code Supplier Size Price
Controllable NOdonor <NO-Rosa5>
DatasheetThis may not be the latest data sheet.
FDV-0032 FNAFunakoshi Co.,Ltd. 0.25 mg $300

Description
Storage -20°C CAS
Link

[Date : December 10 2019 23:16]

Controllable NOdonor <NO-Rosa5>


  • Product Code: FDV-0032
  • Supplier: FNA
  • Size: 0.25mg
  • Price: $300

Description
Storage -20°C CAS
Link

Go back to index

CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.