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HOME> Products> Cell Culture> Medium> HemEx-Type9A
HOME> Products> Cell Culture> Stem cells> HemEx-Type9A

Serum-free medium with PVA for mouse Hematopoietic Stem Cells HemEx-Type9A

Date：September 06 2019Web Page No：81234

Cell Science & Technology Institute, Inc.

Medium for mouse Hematopoietic Stem Cells for maintaining undifferentiated state by Polyvinyl Alcohol (PVA)
HemEx-Type 9A

HemEx-TypeA is a serum-free media, which contains Polyvinyl Alcohol (PVA) and can be used for mouse Hematopoietic Stem Cells (HSCs).

In general, culture media for HSCs contains serum albumin. Such albumin is required to induce cell division of HSCs. On the other hand, albumin inhibits stable undifferentiated state.

Widely used purified or recombinant albumin contains small amount of contaminants, but are tough to remove.
It is reported that such contaminants induce differentiation of HSCs. Moreover, protein's oxidation reaction induces cellular senescence. Thefore, albumin needs to be replaced by other chemicals.

Dr. Satoshi Yamazaki at The Institute of Medical Science, The University of Tokyo reported that PVA can replace albumin (1). Moreover, PVA is a stable chemical with no oxidation reaction in medium so aging of HSCs are also suppressed. These advantages help to do expansion culture of HSCs while maintaining undifferentiated state.

Features
Protocol
Data
Reference
Product Information

Features

Optimized for the expansion of mouse HSCs, Hematopoietic Stem and Progenitor Cell (HSPCs) obtained from bone marrow.
Can do expansion culture of HSCs, for more than 1 month in undifferentiated state.
PVA is used instead of albumin.

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Protocol

Please refer to the paper below:

Long-term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation

Wilkinson, C., et al. Nature, 571, 117～121 (2019).

Note: For culture, addition of cytokine is required.

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Data

Fig. 1 Flow cytometry analysis of HSCs
HSCs were cultured with HemEx supplemented with 10 ug/ml of SCF and 100 ug/ml of TPO for 7 days in 96 well U-bottom culture plate. Lin-/Sca-1+/c-Kit+ is defined as undifferentiated mouse HSPCs in this data.

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Reference

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Product Information

[Date : May 05 2024 00:05]

Detail Product Name Product Code Supplier Size Price

HemEx-Type9A
DatasheetThis may not be the latest data sheet. 87-476 KSKCell Science & Technology Institute, Inc. 100 ml $201

Contact

Description	Mouse HSC expansion basal medium
Storage	4°C,Dark Strage	CAS
Link

[Date : May 05 2024 00:05]

HemEx-Type9A

DatasheetThis may not be the latest data sheet.

Product Code: 87-476
Supplier: KSK
Size: 100ml
Price: $201

Description	Mouse HSC expansion basal medium
Storage	4°C,Dark Strage	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

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