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PolyamineRED | Funakoshi
Intracellular Polyamine Imaging Reagent PolyamineRED | Funakoshi
Date:June 28 2018Web Page No:81084
Funakoshi Co.,Ltd.
![PolyamineRED logo](/domestic/f-img/ds/FNA_67882_logo.jpg)
PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines.
* This product has been commercialized with the support of Biofunctional Synthetic Chemistry Laboratory, RIKEN.
![PolyamineRED cell](/domestic/f-img/ds/FNA_67882_1R.jpg)
Selective Visualization of Intracellular Polyamines with PolyamineRED
What is Polyamine
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Features
- Specifically labels polyamines
- Can be detected by filter set for Rhodamine (Ex/Em : 560nm / 585 nm)
- Cell permeable : compatible with live cell imaging
- No pretreatments required
- Can do semi-quantification of total polyamines amount* by intracellular fluorescence intensity.
* Only total polyamines, not for each polyamine.
Application Data
Polyamine Detection in Cancer or Non-cancer Cells
Three cancer cell lines (MCF7, MDA-MB-231, SK-BR-3) and two non-cancer cells (MCF10A, lymphocyte) were treated with 30 μM of PolyamineRED for 10 min. After incubation, cells were washed three times by PBS, followed by DAPI staining and formalin fixation. TAMRA fluorescence was detected in cancer cell lines significantly. On the other hand, because of the low polyamine levels in non-cancer cells, little TAMRA signal was detected in MCF10A or lymphocytes .
![Polyamine Detection in Cancer or Non-cancer Cells](/domestic/f-img/ds/FNA_67882_5.jpg)
Intracellular Localization of Polyamines
MDA-MB-231 cells were treated with 30 μM of PolyamineRED for 10 min, stained by DAPI and fixed. Major TAMRA signal was detected in nucleus. This indicates polyamines in MDA-MB-231 are mainly localized in nucleus.
![Localization of Polyamines](/domestic/f-img/ds/FNA_67882_6.jpg)
Reference Data: Selectivity of Glycine Propagyl Ester to Polyamines
A model molecule of glycine propagyl ester (benzyloxycarbonyl glycine propagyl ester) was reacted with polyamines (spermine, spermidine and putrescine), amino acids and monoamines, then the reactants were followed by HPLC.
The reaction proceeded only with polyamines (spermine, spermidine, putrescine) and hardly with monoamines (epinephrine) and amino acids (lysine). Reactivity to polyamines tended to increase with the number of amino groups. (citated by original paper)
Substance | Spermine | Spermidine | Putrescine | Epinephrine | Lysine |
---|---|---|---|---|---|
Reaction Rate% | 82% | 78% | 66% | <1% | 2% |
Original Paper
- K. Vong, K. Tsubokura, Y. Nakao, T. Tanei, S. Noguchi, S. Kitazume, N. Taniguchi, K. Tanaka, "Cancer cell targeting driven by selective polyamine reactivity with glycine propargyl esters.", Chem. Commun., 53, 8403~8406 (2017). [PMID : 28589196]
Product Information
[Date : July 27 2024 00:10]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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PolyamineRED, Intracellular Polyamine Detection Reagent DatasheetThis may not be the latest data sheet. |
FDV-0020 | FNAFunakoshi Co.,Ltd. | 0.5 mg | $380 | |||||||||||||||||||||||||||||||
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[Date : July 27 2024 00:10]
PolyamineRED, Intracellular Polyamine Detection Reagent
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0020
- Supplier: FNA
- Size: 0.5mg
- Price: $380
Description |
PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines. |
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Storage | -20°C | CAS | |
Link |
CONTACT
export@funakoshi.co.jp
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