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Intracellular Polyamine Imaging Reagent PolyamineRED | Funakoshi

Date:June 28 2018Web Page No:81084

Funakoshi Co.,Ltd.

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PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines.
* This product has been commercialized with the support of Biofunctional Synthetic Chemistry Laboratory, RIKEN.

PolyamineRED cell

Selective Visualization of Intracellular Polyamines with PolyamineRED

What is Polyamine

The polyamine species, such as putrescine, spermidine and spermine etc. and its acetyl derivatives, are some of the essential class of metabolites which have liner alkyl structure with two or more amines.

Polyamines have polycationic properties and show various biological functions. For example, polyamines interact with DNA and RNA in the nuclear and regulate gene expression. Polyamines also interact with negatively charged proteins and control its function. The major source of polyamines is amino acid ornithine. In case of mammalian, ornithine is converted to putrescine by ornithine decarboxylase (ODC), followed by synthesizing spermindine and spermine. As ODC is highly expressed in cancer cells, polyamines are considered as cancer marker.
Several detection methods of polyamines are developed so far, but, most of them are low-throughput systems using HPLC with polyamine standard compounds. Therefore, biological functions of polyamines have been unclear.
PolyamineRED is the world's first reagent for detecting intracellular polyamines without any pre-treatment and cell lysis. This TAMRA (tetramethylrhodamine)-conjugated derivative of glycine propagyl ester specifically reacts with linear primary alkylamine and has cell-penetrating properties, specifically reacts with polyamines inside the cells and labelled polyamines with red fluorescent dye TAMRA.

PolyamineRED

Polyamine Biosynthesis Pathway in vivo

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Features

  • Specifically labels polyamines
  • Can be detected by filter set for Rhodamine (Ex/Em : 560nm / 585 nm)
  • Cell permeable : compatible with live cell imaging
  • No pretreatments required
  • Can do semi-quantification of total polyamines amount* by intracellular fluorescence intensity.

  • * Only total polyamines, not for each polyamine.

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Application Data

Polyamine Detection in Cancer or Non-cancer Cells

Three cancer cell lines (MCF7, MDA-MB-231, SK-BR-3) and two non-cancer cells (MCF10A, lymphocyte) were treated with 30 μM of PolyamineRED for 10 min. After incubation, cells were washed three times by PBS, followed by DAPI staining and formalin fixation. TAMRA fluorescence was detected in cancer cell lines significantly. On the other hand, because of the low polyamine levels in non-cancer cells, little TAMRA signal was detected in MCF10A or lymphocytes .

Polyamine Detection in Cancer or Non-cancer Cells

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Intracellular Localization of Polyamines

MDA-MB-231 cells were treated with 30 μM of PolyamineRED for 10 min, stained by DAPI and fixed. Major TAMRA signal was detected in nucleus. This indicates polyamines in MDA-MB-231 are mainly localized in nucleus.

Localization of Polyamines

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Reference Data: Selectivity of Glycine Propagyl Ester to Polyamines

A model molecule of glycine propagyl ester (benzyloxycarbonyl glycine propagyl ester) was reacted with polyamines (spermine, spermidine and putrescine), amino acids and monoamines, then the reactants were followed by HPLC.
The reaction proceeded only with polyamines (spermine, spermidine, putrescine) and hardly with monoamines (epinephrine) and amino acids (lysine). Reactivity to polyamines tended to increase with the number of amino groups. (citated by original paper)

Substance Spermine Spermidine Putrescine Epinephrine Lysine
Reaction Rate% 82% 78% 66% <1% 2%

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Original Paper

  • K. Vong, K. Tsubokura, Y. Nakao, T. Tanei, S. Noguchi, S. Kitazume, N. Taniguchi, K. Tanaka, "Cancer cell targeting driven by selective polyamine reactivity with glycine propargyl esters.", Chem. Commun., 53, 8403~8406 (2017). [PMID : 28589196]

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Product Information

[Date : June 20 2024 00:07]

Detail Product Name Product Code Supplier Size Price
PolyamineRED, Intracellular Polyamine Detection Reagent
DatasheetThis may not be the latest data sheet.
FDV-0020 FNAFunakoshi Co.,Ltd. 0.5 mg $380

Description PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines.
Storage -20°C CAS
Link

Lipid Peroxidation Detection Tools for Cancer Research

[Date : June 20 2024 00:07]

PolyamineRED, Intracellular Polyamine Detection Reagent


  • Product Code: FDV-0020
  • Supplier: FNA
  • Size: 0.5mg
  • Price: $380

Description PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines.
Storage -20°C CAS
Link

Lipid Peroxidation Detection Tools for Cancer Research

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export@funakoshi.co.jp

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