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Washable Cell-Staining Probe CytoSeeing, Reversible Cytoplasm Blue

Date:March 14 2018Web Page No:81015

Funakoshi Co.,Ltd.

CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.

This product has been commercialized based on the research result of Hokkaido University Faculty of Science.

Washable Staining Image

Washable staining image

This reagent stains cytoplasmic and endomembrane system but not nucleus. The dye can be removed easily by washing step with medium change and is useful for observation of transient cell morphology.

Background

It is well known that morpholgy change of nuclear and cytoplasm are related to differentiation, function and signal response of cells. Conventional cytoplasm staining dyes are used for cell tracking. Therefore, once dyes are incorporated into cells, the dyes remain in cytoplasm even after medium change. The dyes are diluted in the course of cell division, and disappear after 3 to 6 population doubling. If dyes remained in the cells, it is difficult to observe the cell with other probes after live cell imaging.

CytoSeeing differs from conventional reagents, it can stain only cytoplasm and wash it away.

Reference

  • Kamada, R. et al., "Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells", PLOS ONE, 11(8):e0160625 (2016). [PMID:27490470]
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Features

  • Easy protocol: Just add CytoSeeing to culture medium
  • No staining on nucleus
  • Compatible with green and red fluorescent dyes
  • Little effect to cell function
  • Removable: Just replace with fresh medium without containing CytoSeeing. Cells can be used for further assays
  • Compatible with both adhesive and suspension cells

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Comparison with Other Cytoplasm Staining Dye

Reagent Cytoplasm Staining Visible Nuclear Morphology Introduction Time Wash-out
CytoSeeing Good Good 3 - 9 min Good
Company T Good Bad 15 min - 1 hour Bad
Company A Good Bad 15 min - 1 hour Bad

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Product Summary

  • Molecular Formula : C17H12N3
  • Molecular Weight : 258.11
  • Purity : ≥97%
  • No effect to cell function
  • Ex/Em : 345 nm / 456 nm
  • Solubility : DMSO*

* 10 mM stock solution is recommended.

CytoSeeing Spectral Diagram

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Protocol

  1. Culture cells
  2. Add CytoSeeing solution to medium, with the final concentration from 10 μM to 50 μM.
  3. Incubate for a few minutes.
  4. Observe cells by fluorescent microscopy.
  5. If needed, remove CytoSeeing by replacing with fresh medium (without containing CytoSeeing).

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Example Data

CHO cells Stained with CytoSeeing

CHO cells were treated with CytoSeeing (10 μM) for 30 min. The entire cytoplasm is stained, and the nucleus morphology can also be confirmed by observing nuclear boundary.

細胞増殖阻害試験
細胞増殖阻害試験

Incorporation & Distribution of CytoSeeing in A549 cells by Time Course

Incorporation and distribution of <b>CytoSeeing</b> in A549 cells by time course
  • a) CytoSeeing (10 μM) was added and incubated. CytoSeeing was completely incorporated into cell by 9 minutes.
  • b) CytoSeeing incorporated cells were washed and incubated in fresh medium (without CytoSeeing). Almost CytoSeeing was removed in 30 minutes.

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Product Information

[Date : June 20 2024 00:07]

Detail Product Name Product Code Supplier Size Price
CytoSeeing, Reversible Cytoplasm Blue
DatasheetThis may not be the latest data sheet.
FDV-0017 FNAFunakoshi Co.,Ltd. 1 mg $350

Description CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.
Storage -20°C CAS
Link

"CytoSeeing" (Reversible cytoplasm blue fluorescent dye) in a minute!

[Date : June 20 2024 00:07]

CytoSeeing, Reversible Cytoplasm Blue


  • Product Code: FDV-0017
  • Supplier: FNA
  • Size: 1mg
  • Price: $350

Description CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.
Storage -20°C CAS
Link

"CytoSeeing" (Reversible cytoplasm blue fluorescent dye) in a minute!

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CONTACT

export@funakoshi.co.jp

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  • Please note that Product Information or Price may change without notice.