Washable Cell-Staining Probe CytoSeeing™ (Reversible Cytoplasm Blue)
Date：March 14 2018Web Page No：81015
Funakoshi Co.,Ltd.
CytoSeeing™ is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing™.
※ This product has been commercialized based on the research result of Hokkaido University Faculty of Science.
- Background
- Features
- Comparison with Other Cytoplasm Staining Dye
- Product Summary
- Protocol
- Example Data
- Webinar Movie
- Product Information
Background
It is well known that morpholgy change of nuclear and cytoplasm are related to differentiation, function and signal response of cells. Conventional cytoplasm staining dyes are used for cell tracking. Therefore, once dyes are incorporated into cells, the dyes remain in cytoplasm even after medium change. The dyes are diluted in the course of cell division, and disappear after 3 to 6 population doubling. If dyes remained in the cells, it is difficult to observe the cell with other probes after live cell imaging.
CytoSeeing™ differs from conventional reagents, it can stain only cytoplasm and wash it away.
Reference
- Kamada, R. et al., "Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells", PLOS ONE, 11(8)：e0160625 (2016). [PMID：27490470]
Features
- Easy protocol: Just add CytoSeeing™ to culture medium
- No staining on nucleus
- Compatible with green and red fluorescent dyes
- Little effect to cell function
- Removable: Just replace with fresh medium without containing CytoSeeing™. Cells can be used for further assays
- Compatible with both adhesive and suspension cells
Comparison with Other Cytoplasm Staining Dye
| Reagent | Cytoplasm Staining | Visible Nuclear Morphology | Introduction Time | Wash-out |
|---|---|---|---|---|
| CytoSeeing™ | Good | Good | 3 - 9 min | Good |
| Company T | Good | Bad | 15 min - 1 hour | Bad |
| Company A | Good | Bad | 15 min - 1 hour | Bad |
Product Summary
Protocol
- Culture cells
- Add CytoSeeing™ solution to medium, with the final concentration from 10 μM to 50 μM.
- Incubate for a few minutes.
- Observe cells by fluorescent microscopy.
- If needed, remove CytoSeeing™ by replacing with fresh medium (without containing CytoSeeing™).
Example Data
CHO cells Stained with CytoSeeing™
CHO cells were treated with CytoSeeing™ (10 μM) for 30 min. The entire cytoplasm is stained, and the nucleus morphology can also be confirmed by observing nuclear boundary.
Incorporation & Distribution of CytoSeeing™ in A549 cells by Time Course
- a) CytoSeeing™ (10 μM) was added and incubated. CytoSeeing™ was completely incorporated into cell by 9 minutes.
- b) CytoSeeing™ incorporated cells were washed and incubated in fresh medium (without CytoSeeing™). Almost CytoSeeing™ was removed in 30 minutes.
Webinar Movie
Product Information
[Date : April 25 2025 00:06]
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CytoSeeing, Reversible Cytoplasm Blue DatasheetThis may not be the latest data sheet. |
FDV-0017 | FNAFunakoshi Co.,Ltd. | 1 mg | $350 | |||||||||||||||||||||||||||||||
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[Date : April 25 2025 00:06]
CytoSeeing, Reversible Cytoplasm Blue
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0017
- Supplier: FNA
- Size: 1mg
- Price: $350
| Description |
CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing. |
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| Storage | -20°C | CAS | |
| Link |
"CytoSeeing" (Reversible cytoplasm blue fluorescent dye) in a minute! |
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CONTACT
export@funakoshi.co.jp
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- ※Please note that Product Information or Price may change without notice.