HOME> Products> Protein / Enzyme> New type Protein Transfection Reagent : ProteoCarry

New type Protein Transfection Reagent : ProteoCarry

Date:November 28 2017Web Page No:80968

Funakoshi Co.,Ltd.

Going to Cytoplasm, not ending up in endosome!
ProteoCarry

ProteoCarry is a new type peptide base transfection reagent.

When using a conventional protein transfection reagent, transfected proteins are accumulated in endosome or lysosome, and most of such proteins are dissociated in lysosome proteolysis system.
Contrary, ProteoCarry can deliver transfected proteins to cytoplasm effectively.
Moreover, pre-incubation with proteins are NOT needed.
Therefore effect to the protein functions can be minimized.

Features

  • Highly soluble in water
  • High efficiency of delivering proteins to cytoplasm
  • Fast - 1 hour incubation
  • No pre-incubation with proteins needed
  • Effect to the function of proteins cab be minimized
  • Low cytotoxicity
  • Polysaccharide can be also transfected.

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Kit components

  • ProteoCarry, 4mg in vial (vial A)
  • FITC-dextran as positive control, 2 mg in vial (vial B)

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Storage

  • Shipping : Room temperature
  • Store : -20℃

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Validated cell types

  • HeLa, SW280, COS7, NIH3T3, HUVEC<>li/

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Procedure overview

Procedure overview

[Date : August 13 2018 23:10]

Detail Product Name Product Code Supplier Size Price
ProteoCarry <Protein Transfection Reagent>
Datasheet
FDV-0015 FNAFunakoshi Co.,Ltd. 1 set $400

Description ProteoCarry is a new type peptide base transfection reagent. When using a conventional protein transfection reagent, transfected proteins are accumulated in endosome or lysosome, and most of such proteins are dissociated in lysosome proteolysis system. Contrary, ProteoCarry can deliver transfected proteins to cytoplasm effectively. Moreover, pre-incubation with proteins are NOT needed. Therefore effect to the protein functions can be minimized.
Storage -20°C CAS
Link

[Date : August 13 2018 23:10]

ProteoCarry <Protein Transfection Reagent>


  • Product Code: FDV-0015
  • Supplier: FNA
  • Size: 1set
  • Price: $400

Description ProteoCarry is a new type peptide base transfection reagent. When using a conventional protein transfection reagent, transfected proteins are accumulated in endosome or lysosome, and most of such proteins are dissociated in lysosome proteolysis system. Contrary, ProteoCarry can deliver transfected proteins to cytoplasm effectively. Moreover, pre-incubation with proteins are NOT needed. Therefore effect to the protein functions can be minimized.
Storage -20°C CAS
Link

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About protein transfection reagent and issue of conventional one

Protein transfection reagent has been widely used as it can deliver proteins faster than inducing proteins by gene transfer.
Many reagents have been developed so far, such as peptide-base, cationic lipid-base, and polymer base. Proteins transfected by such reagents generally form complex with proteins and incorporated to cells by endocytosis. After that, the complex breaks endosome membrane and transported to cytoplasm.

However, current these reagents have weak activity for disrupting membranes.
Therefore, such arrested proteins cannot escape from endosome. Arrested proteins are considered to move to lysosome proteolysis system.
Therefore, transfection reagents should have strong activity for disrupting membranes so that transfected proteins could be functioned and transported in cytoplasm effectively.

ProteoCarry, a new peptide-base transfection reagent has a strong and selective membrane-disrupting activity. ProteoCarry does not require a forming of protein-reagent complex. Therefore, not only proteins, but also biopolymers can also be transfected to cells.

About protein transfection reagent and issue of conventional one

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CONTACT

export@funakoshi.co.jp

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