MRMplus Retention Time Marker
Date:June 21 2017Web Page No:80768
MRMplus Retention Time Marker is the mixture of marker peptides (12 synthetic peptides) for calculating the retention times of LC in LC-MS/MS system for scheduled-MRM. The segment value of each MRMplus Standard Peptide is easily converted by using this marker and the conversion software.
Component
200 x MRMplus Retention Time Marker Stock Solution (12 µl)
*Dilute 1 µl of Stock Solution into 199 µl of sample diluent for your LC system as Working Solution. When you use TFA as the sample diluent, grass ampule type product (eg. #28902 from Thermo Scientific) is recommended. Working Solution should be stored at 4ºC
NOTE: AVOID FREEZE-THAW CYCLES for the stock solution
NOTE: Please use working solution within ONE WEEK
General Protocol
*This protocol is optimized for QTRAP5500 System (nanoESI, ABSciex). For the other LC-MS/MS systems, optimal condition should be determined by users.
- Inject 0.2 µl of the Working Solution into LC.
- Optimize LC conditions and injection volume.
- Input the values of Q1 and Q3 for 12 marker peptides and conduct MRM analysis without scheduling function.
- Write down the detected retention time of 12 marker peptides into spread sheet.
- Convert segment values of internal standard peptides specific to your target proteins into expected retention time using the detected retention time and conversion software (RTimeToSegment.exe).
- Input the expected retention time into your LC-MS/MS system.
- Mix 1 µl Retention Time Marker for 1 µg sample, and then add the suitable amount of internal standard peptide. Dilute the mixture to 10 µl with sample diluent, use 2 µl for conduct scheduled MRM analysis.
NOTE: You can find the information of the Q1, Q3 values, and conversion software (RTimeToSegment.exe) on FUNAKOSHI website (http://www.funakoshi.co.jp/contents/7979).
NOTE: You should set a wide range of expected retention time for scheduling, or you would miss the target peptide.
NOTE: Some marker peptides may appear closely each other or sometimes interchanged depending on your LC conditions, such as gradient, colums used, or room temperature.
Reference
- A large-scale targeted proteomics assay resource based on an in vitro human proteome, Nature Methods, 10.1038/nmeth.4116
[Date : February 15 2024 12:37]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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MRMplus Retention Time Marker DatasheetThis may not be the latest data sheet. |
FMR-002 | FMRFunakoshi Co.,Ltd. | 12 µl | $150 | |||||||||||||||||||||||||||||||
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[Date : February 15 2024 12:37]
MRMplus Retention Time Marker
DatasheetThis may not be the latest data sheet.
- Product Code: FMR-002
- Supplier: FMR
- Size: 12µl
- Price: $150
Description |
MRMplus Retention Time Marker is the mixture of marker peptides (12 synthetic peptides) for calculating the retention times of LC in LC-MS/MS system for scheduled-MRM. The segment value of each MRMplus Standard Peptide is easily converted by using this marker and the conversion software. |
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Storage | -80°C | CAS | |
Link |
Quantitative proteomics tools & analysis service by iMPAQT method |
CONTACT
export@funakoshi.co.jp
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