HOME> Products> Miscellaneous> Exosome Purification Column : EVSecond

Exosome Purification Column : EVSecond

Date:February 16 2017Web Page No:80766

Exosome purification column by size exclusion chromatography EVSecond

EVSecond is a specially developed size exclusion chromatography (SEC) column, for Exosome purification.
Exosome can be easily purified from serum, plasma and cell culture supernatant.
Special instrument, such as ultracentrifuge is not required.

Features

  • Easy procedure by free fall
    - Special instruments are not required.
  • Best for exhaustive miRNA analysis and high sensitivity proteome analysis
    - Free RNA or protien in sample can be efficiently removed.
  • Exosome can be applied to further application
    - Purified exosome do not have structural damage.
  • Special rack "GL-SPE EXO Fraction Rack" is available as an option
    - This special rack makes preparation and collection easy.

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Example Data

Example Data

Fig. 1 Purification of exosome from human serum
Each 100 μL of fraction are collected. By using EVSecond, exosome were purified from 100, 200, 300 or 700 μL human serum.
Exosome (Red) and serum proteins (Blue, e.g. albumin or IgG) are separated accurately.
Red : Exosome fraction site was confirmed by CD9 sandwich ELISA.
Blue : Serum proteins fraction site was confirmed by Bradford method.
Data is kindly provided by Dr. Ueda (University of Tokyo)

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Procedure

(A) Preparation of Column (To be executed in 4 degree condition)
Mix filler well by inverting column until filler is mixed evenly and completely.
* Mix the column on the shaker overnight gently in cold room is recommended.
* Filler clumps may cause incorrect fraction. Check no clumps are found before use.

(B) Purification (To be executed in 4 degree condition)

  1. Remove two caps (top and bottom) and discard storage buffer.
  2. Block the filler by 700 μL of FBS (filtrated by 0.22 μm)
  3. Wash 6 times by 700 μL of PBS
  4. Apply 50 to 700 μL of sample. Flow all sample completely.
  5. Apply 100 μL of PBS twice
  6. Apply 750 to 1000 μL of PBS (total added volume from 4 to 6 should be 1000 μL). Flow all volume and discard it.
  7. Prepare collection tube, and collect 12 fractions by 100 μL of PBS.

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Attention

* Initially, elution position of exosome and serum proteins should be checked by ELISA (CD9 ELISA, for exosome) or Bradford method (for serum proteins).
* Samples which contain objects and may stuck in the column should be pretreated by following protocol.

  1. Centrifuge sample by 12000 x g, for 15 minutes in 4℃.
  2. Collect sample from middle layer (50 to 700 μL). Do not take precipitation at the bottom or lipids (upper layer).

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Special Rack : GL-EXO Fraction Rack

Special Rack : GL-EXO Fraction Rack
  • Rack can hold up to 8 collection columns (1.5 mL or 2 mL).
  • Collection columns can be shifted by manipulating a lever.
  • Up to six sets of columns can be set.
  • Size : 300(W)×300(D)×150(H) mm

[Date : January 25 2020 00:22]

Detail Product Name Product Code Supplier Size Price
EVSecond
501021390 GLI 10 pieces $500

Description
Storage CAS
Link

EVSecond
501021392 GLI 25 pieces $1,100

Description
Storage 4°C CAS
Link

GL-SPE EXO Fraction Rack
501050450 GLI 1 set $1,400

Description
Storage CAS
Link

[Date : January 25 2020 00:22]

EVSecond


  • Product Code: 501021390
  • Supplier: GLI
  • Size: 10pieces
  • Price: $500

Description
Storage CAS
Link

EVSecond


  • Product Code: 501021392
  • Supplier: GLI
  • Size: 25pieces
  • Price: $1,100

Description
Storage 4°C CAS
Link

GL-SPE EXO Fraction Rack


  • Product Code: 501050450
  • Supplier: GLI
  • Size: 1set
  • Price: $1,400

Description
Storage CAS
Link

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CONTACT

export@funakoshi.co.jp

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  • Please note that Product Information or Price may change without notice.