Exosome Purification Column : EVSecond L70
Date：February 16 2017Web Page No：80766
Exosome purification column by size exclusion chromatography EVSecond L70
EVSecond is a specially developed size exclusion chromatography (SEC) open column, for exosome purification.
exosomes can be easily purified from serum, plasma and cell culture supernatant.
Special instruments, such as ultracentrifuge is not required.
Features
- Easy procedure by free fall
- Special instruments are not required. - Best for exhaustive miRNA analysis and high sensitivity proteome analysis
- Free RNA or protein in sample can be efficiently removed. - Exosome can be applied to further application
- Purified exosome do not have structural damage. - Special rack "GL-SPE EXO Fraction Rack" is available as an option
- This special rack makes preparation and collection easy.
Example Data
Fig. Purification of exosomes from human serum
Isolationg exosomes from 200 μL of human serum (100 μL / fraction)
Red line: CD9-CD9 xosome sandwich ELISA (detecting exosomes)
Blue line: Bradford assay (detecting serum free proteins)
Procedure
(A) Preparation of Column (To be executed in RT condition)
Mix filler well by inverting column until filler is mixed evenly and completely.
* Mix the column several minutes gently in RT is recommended.
* Filler clumps may cause incorrect fraction. Check no clumps are found before use.
(B) Purification (To be executed in RT condition)
- Remove two caps (top and bottom) and discard storage buffer.
- Block the filler by 700 μL of FBS (filtrated by 0.22 μm)
- Wash 3 times by 1500 μL of PBS
- Apply 50 to 1500 μL of sample. Flow all sample completely.
- Flow proper amount of PBS
- Collect exosomes fraction
Attention
* Initially, elution position of exosome and serum proteins should be checked by anti-CD9-anti-CD63 sandwich ELISA (for exosome) or anti-CD9 western blot (for serum proteins).
Special Rack : GL-EXO Fraction Rack
(1)Column hanger
Maxium 6 columns can be installed.
(2)Collection tube racks
Maxium 8 collection tubes(1.5 mL or 2 mL) can be set.
The collectcion tube can be switched by pulling the lever.
(3)Base plate
Maximum 6 collection tube racks can be set.
Demension (for EVSecond L70): 300(W)x300(D)x189(H) mm
Demention(for EVSecond): 300(W)x300(D)x150(H) mm
[Date : February 15 2024 12:37]
[Date : February 15 2024 12:37]
CONTACT
export@funakoshi.co.jp
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