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Even 10 seconds! Kaneka High-Speed DNA Polymerase

Date：February 07 2017Web Page No：80762

Shortening your PCR time!
Kaneka High-Speed DNA Polymerase

Kaneka High-Speed DNA Polymerase shortens extension time of PCR.

Features

  • Excellent DNA amplification efficiency
    - Kaneka High-Speed DNA Polymerase can amplify DNA from a tiny amount of template DNA.
    Therefore, it is suitable for not only regular PCR, but also colony PCR or detection of microbe.

  • High speed DNA amplification
    - Extension time is 5 seconds/1kb (under 2kb amplification condition).

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Kit contents

  • Kaneka High-Speed DNA Polymerase (50 μL x 1)
  • 10X Kaneka High-Speed DNA Polymerase Buffer (500 μL x 1)
  • 2 mM dNTPs (500 μL x 1)

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Example Data

Fig.1 PCR using λDNA as a template DNA

Fig.1 PCR using λDNA as a template DNA
Kaneka High-Speed DNA Polymerase can amplify DNA under extension time 5 seconds protocol. However, other polymerase cannot do it.

Fig.2 Tolerance to impurities in sample

Fig.2 Tolerance to impurities in sample
DNA from plants are extracted and amplified using Kaneka High-Speed DNA Polymerase or others.
Kaneka High-Speed DNA Polymerase can amplify DNA from impure samples.

1.Tomato seed
2.Orchid leaf
3.Wheat seed
4.Corn seed
5.Water melon seed
6.Peanut seed
7.Buckwheat seed
8.Apple seed
9.Mandarin orange seed
10.Banana pulp

Fig. 3 Amplification with an extension time of 30 seconds / 1kb

Fig. 3 Amplification with an extension time of 30 seconds / 1kb
Reaction mixture was prepared using λ DNA (6.7 ng) as a template in accordance with the "PCR reaction mixture preparation example" above and PCR was performed using T3000 Thermocycler (Biometra). Extension time : 30 seconds was applied when the amplification length was shorter than 1 kb, or 30 seconds / 1 kb when the amplification length was longer than 1 kb.

Fig. 4 Amplification using with an extension time of 5 seconds / 1kb

Fig. 4 Amplification using with an extension time of 5 seconds / 1kb
Reaction mixture was prepared using λ DNA (6.7 ng) as a template in accordance with the "PCR reaction mixture preparation example" above and PCR was performed using T3000 Thermocycler (Biometra).
Extension time : 5 seconds was applied when the amplification length was shorter than 1 kb,
or 5 seconds / 1 kb when the amplification length was longer than 1 kb.

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price

[Date : February 15 2024 12:37]

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CONTACT

export@funakoshi.co.jp

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