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Monoclonal Antibody for Scirrhous Stomach Cancer Anti-MUC1 Antibody (014E)

Date：November 22 2016Web Page No：80739

Funakoshi Co.,Ltd.

This antibody can detect the tumor marker MUC1 (Mucin-1; also PEM, PEMT and Episialin) in various tumor tissues by immunohistochemistry. In particular, it can specifically detect scirrhous gastric cancer, which has been considered difficult to detect.
※ This antibody has been commercialized under a license from Kagoshima University.

MUC1(Mucin1)IHC

IHC of human gastric carcinoma tissue

  • Sample: Human stomach cancer
  • Detection reagent: ImmPRESSReagent, Anti-Mouse IgG (Vector Laboratories #MP-7402-15)

MUC1(Mucin1)West

WB of cell lysate (5 μg)

  • Left: Capan-1 (Human pancreas cell line: MUC1 positive)
  • Right: HEK293 (MUC1 negative)

MUC1 & MUC1 Antibody(014E)

MUC1 (Mucin1), a mucin-type glycoprotein, is expressed in various cancers and is thought to be involved in cancer cell invasion and metastasis.

MUC1 is expressed from a single gene as a heterodimer of the extracellular region (MUC1-N) and the extracellular to transmembrane to cytoplasmic region (MUC1-C). MUC1-N contains a tandem repeat sequence (O-glycosylated site), and glycosylation is performed on this region. In some cases, MUC1-N is cleaved. On the other hand, MUC1-C has many splicing variants.

Most conventional MUC1 antibodies used MUC1-N or cleavage sites as immunogen, therefore sometimes it could not detect MUC1-N depending on its cleavage or glycosylation status. The clone 014E is raised against common region among splicing variants in the cytoplasmic tail domain (CTD) of MUC1-C. Therefore, it is possible to detect various splicing variants, regardless of MUC-1's structure of extracellular region.

Mucin-1 gene expression

Detection of gastric cancer including scirrhous

IHC with antibodies (e.g. MUC1 antibody, keratin antibody), HE stain and PAS stain with diastase are used to detect stomach cancer.

However, these detection methods may miss cancer cells due to no staining of scirrhous gastric carcinoma (signet ring cell carcinoma: sig, poorly differentiated adenocarcinoma non-solid type: por2), unclear images and non-specific staining. The clone 014E antibody can specifically detect even scirrhous gastric cancer, which has been considered difficult to detect 1, 5.

Detection of pancreatic cancer

In pancreatic cancer detection, some clones of MUC1 antibody could only detect pancreatic ductal adenocarcinoma (PDAC) and could not detect intraductal papillary mucinous tumor (IPMN-gastric type and IPMN-intestinal type). This is because cleavage or glycosylation status of MUC1-N varies in these tumors, and antibodies with the extracellular region as antigen could not detect them.

Since clone 014E antibody is raised against cytoplasmic region, it can detect MUC1-N regardless of MUC1' structure of the extracellular region 4, 5. Also, a correlation between the MUC1 detection results by clone 014E and methylation of promoter region of MUC1 gene has been reported 4, 5.

参考文献

  1. Yonezawa, S., et al.,"A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach", Gastric Cancer, 15(4), 370～381 (2012) [PMID: 22237656].
  2. Yokoyama, S., et al., "The application of methylation specific electrophoresis (MSE) to DNA methylation analysis of the 5' CpG island of mucin in cancer cells", BMC Cancer, 12, 67 (2012). [PMID: 22329852].
  3. Kitamoto, S., et al., "MUC1 enhances hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors", Oncogene, 32(39), 4614～4621 (2013). [PMID: 23108411].
  4. Yokoyama, S., et al., "Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice", PLoS ONE, 9(4):e93760 (2014). [PMID: 24714692].
  5. Japan Patent 5660486, Patent US8951526 B2

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Features

  • The majority of splicing variants can be detected regardless of the structure of the extracellular region of MUC1.
  • Isotype : IgG1κ
  • Immunogen : Synthetic Peptide, common region in Cytoplasmic Tail Domain (CTD) of human MUC1 (CRYVPPSSTDRSPYEKVSAG)
  • Reactivity : Human
  • Specificity : Human Mucin1 (MUC1)
  • Application : Immunohistochemistry (Paraffin, 1:2,500 - 1:5,000), Western Blotting (1:2,500 - 1:5,000)
  • Form : Mouse Ascites diluted with PBS

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Product Information

[Date : April 28 2024 00:05]

Detail Product Name Product Code Supplier Size Price

DatasheetThis may not be the latest data sheet. 		FDV-0012B FNAFunakoshi Co.,Ltd. 100 µl $400

Description This antibody can specifically detect scirrhous gastric cancer, which has been considered difficult to detect, and pancreatic cancer, a type of cancer that cannot be detected by some antibodies. Alias: PEM, PEMT, Episialin Clone: 014E
Storage -80°C
Antigen Species Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human Application IHC,Western Blot
Clonality Format Purification Acsites Absorption
Link

[Date : April 28 2024 00:05]

DatasheetThis may not be the latest data sheet.


  • Product Code: FDV-0012B
  • Supplier: FNA
  • Size: 100µl
  • Price: $400

Description This antibody can specifically detect scirrhous gastric cancer, which has been considered difficult to detect, and pancreatic cancer, a type of cancer that cannot be detected by some antibodies. Alias: PEM, PEMT, Episialin Clone: 014E
Storage -80°C
Antigen Species Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human Application IHC,Western Blot
Clonality Format
Purification Acsites Absorption
Link

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CONTACT

export@funakoshi.co.jp

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