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Metallo Assay Copper (Cu) Low Concentrate Assay kit LS – DiBr-PAESA method

Date:June 11 2020Web Page No:80737

Metallo Assay Copper Low Concetrate Assay Kit LS (DiBr-PAESA Method) can measure Copper ion (Cu2+, Cu+) in the biological samples such as Urine saliva, and wide range of samples by microplate reader.

Features

  • Using microplate reader for assay, no need to use Atomic Absorption Spectrometry or ICP-OES Optical Emission Spectrometry, which have been mainly used so far.
  • Good correlation with the result of Atomic Absorption Spectrometry and ICP-OES.
  • High throughput measurement by using microplate reader.
  • Samples from any animal species can be measured.
  • Assay time : 10 minutes
  • 1 point calibration with blank – do not need multiple point measurement to make a standard curve (r^2=0.9999).
  • Kit does not contain any hazardous components like cyanide or azide as preservative.
  • Samples : Urine, Saliva, Cell lysate, Tissue extract, etc

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Copper (Cu)

The main role of proteins which contain Copper (Cu) is oxidation-reduction function. Most enzymes which contain copper directly react with free-oxygen. 95% of copper in plasma binds to oxidative enzyme, that has alpha-2 globulin, ceruloplasmin and ferroxidase activity.

It is reported that heart-related disease, brittle-bone disease, osteoarthritis, Menkes syndrome, and Wilson's Disease are caused by deficiency of copper. On the other hand, excess copper concentration is also toxic.


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Assay Principle

This kit measures copper concentration by color change caused by chelate complex formulation of 3-5 DiBr-PAESA and copper. Copper combined to protein ceruloplasmin is denatured by weak acid and denaturant and then formulates Copper- 3-5 DiBr-PAESA chelate complex. Measuring this complex by 582 nm wavelength and obtain copper concentration.

Assay Principle of Metallo Assay Copper (Cu) Low Concentrate Assay kit LS – DiBr-PAESA method

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Sample preparation

Sample preparation of Metallo Assay Copper (Cu) Low Concentrate Assay kit LS – DiBr-PAESA method
  1. Samples which do not require preparation
    e.g. Urine

    * EDTA treated sample is not suitable for this assay.
    * For pooled urine sample, use HCl addition preserving method. Adjust pH 2 – 3 for assay.

  2. Samples which require acid extraction
    e.g. cell lysate, tissue homogenate and other liquid samples.

    * Measurable chemical species is limited as follows:
    ・ Free copper
    ・ Copper binds to protein (Protein-Cu2+, e.g. albumin, metallothionein)
    * Lysis buffer contains EDTA is not suitable for this assay.
    * Organo-copper and Heme-copper require acidolysis.

  3. Microwave method and samples which require acidolysis
    e.g samples which contain strong chelate agents such as EDTA, Organo-copper (in the case of single bond -C-Cu-C-), copper in cyclic ligand (Cu-Porphyrin)

    * After acidolysis, adjust sample pH in 2 to 3.

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Handling procedure

1. Apply DW, Standard and Sample (12 uL) to 96 well plate. 1. Apply DW, Standard and Sample (12 uL) to 96 well plate.
2. Add working reagent (140 uL). 2. Add  working reagent (140 uL).
3. Incubate at room temperature for 10 minutes 3. Incubate at room temperature for 10 minutes
4. Read the absorbance at 582 nm. 4. Read the absorbance at 582 nm.

5. Calculate concentration
ΔODStd = ODStd – ODBl, ΔODS = ODS – ODBl
Copper (μg/dL) = ΔODS/ΔODStd x 40

* ODS : ODsample, ODBl : ODblank, ODStd : ODstandard

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Citation

  • A novel method of preparing the monoform structure of catalytic antibody light chain  The FASEB Journal ; November 2, 2015
  • Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes. J Life Sci ; June, 2014
  • Cuprizone short-term exposure: astrocytic IL-6 activation and schizophrenia-like behavior. J Pharmacol Sci ; March 15, 2013

[Date : September 24 2020 23:16]

Detail Product Name Product Code Supplier Size Price
Copper, Low Concentrate, Assay Kit
DatasheetThis may not be the latest data sheet.
CU20ME AKJMetallogenics Co.,Ltd. 1 kit $520

Description Metallo Assay Copper Low Concentratekit LS is intended for the quantitative determination of copper in urine or saliva by 96-well reader use.
Storage 4°C CAS
Link

[Date : September 24 2020 23:16]

Copper, Low Concentrate, Assay Kit


  • Product Code: CU20ME
  • Supplier: AKJ
  • Size: 1kit
  • Price: $520

Description Metallo Assay Copper Low Concentratekit LS is intended for the quantitative determination of copper in urine or saliva by 96-well reader use.
Storage 4°C CAS
Link

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CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.