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Metallo Assay Copper (Cu) Assay kit LS – DiBr-PAESA method

Date:June 11 2020Web Page No:80736

Metallo Assay Copper (Cu) Assay kit LS

Metallo Assay Copper Assay Kit LS (DiBr-PAESA Method) can measure Copper ion (Cu2+, Cu+) in the biological samples such as Plasma, Serum, Urine, and wide range of samples by microplate reader.

Features

  • Using microplate reader for assay, no need to use Atomic Absorption Spectrometry or ICP-OES Optical Emission Spectrometry, which have been mainly used so far.
  • Good correlation with the result of Atomic Absorption Spectrometry and ICP-OES.
  • High throughput measurement by using microplate reader.
  • Samples from any animal species can be measured.
  • Assay time : 10 minutes
  • 1 point calibration with blank – do not need multiple point measurement to make a standard curve (r^2=0.9999).
  • Kit does not contain any hazardous components like cyanide or azide as preservative.
  • Samples : Serum, Plasma, Urine*, Saliva, Cell lysate, Tissue extract, Hair extract, Water, etc.

* Please note sample concentration and assay range. If the concentration is low, use #CU20ME, the kit for urine sample.


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opper (Cu)

The main role of proteins which contain Copper (Cu) is oxidation-reduction function. Most enzymes which contain copper directly react with free-oxygen. 95% of copper in plasma binds to oxidative enzyme, that has alpha-2 globulin, ceruloplasmin and ferroxidase activity.

It is reported that heart-related disease, brittle-bone disease, osteoarthritis, Menkes syndrome, and Wilson's Disease are caused by deficiency of copper. On the other hand, excess copper concentration is also toxic.


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Assay Principle

This kit measures copper concentration by color change caused by chelate complex formulation of 3-5 DiBr-PAESA and copper. Copper combined to protein ceruloplasmin is denatured by weak acid and denaturant and then formulates Copper- 3-5 DiBr-PAESA chelate complex. Measuring this complex by 580 nm wavelength and obtain copper concentration.

Assay Principle of Metallo Assay Copper (Cu) Assay kit LS

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Sample Preparation

Sample Preparation of Metallo Assay Copper (Cu) Assay kit LS
  1. Samples which do not require preparation
    e.g. Serum, Plasma

    * EDTA treated sample is not suitable for this assay.

  2. Samples which require acid extraction
    e.g. cell lysate, tissue homogenate and other liquid samples.

    * Measurable chemical species is limited as follows:
    ・Free copper
    ・Copper binds to protein (Protein-Cu2+, e.g. albumin, metallothionein)
    * Lysis buffer contains EDTA is not suitable for this assay.
    * Organo-copper and Heme-copper require acidolysis.

  3. Microwave method and samples which require acidolysis
    e.g samples which contain strong chelate agents such as EDTA, Organo-copper (in the case of single bond -C-Cu-C-), copper in cyclic ligand (Cu-Porphyrin)

    * After acidolysis, adjust sample pH in 2 to 3.

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Handling Procedure

1. Apply DW, Standard and Sample (12 uL) to 96 well plate. 1. Apply DW, Standard and Sample (12 uL) to 96 well plate.
2. Add working reagent (240 uL). 2. Add  working reagent (240 uL).
3. Incubate at room temperature for 10 minutes 3. Incubate at room temperature for 10 minutes
4 .Read the absorbance at 580 nm. 4 .Read the absorbance at 580 nm.

5 .Calculate concentration
ΔODStd = ODStd – ODBl, ΔODS = ODS – ODBl Copper (μg/dL) = ΔODS/ΔODStd x 200

* ODS : ODsample, ODBl : ODblank, ODStd : ODstandard

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Citation

  • Metal Ion Release of Manufactured Metal Oxide Nanoparticles Is Involved in the Allergic Response to Inhaled Ovalbumin in Mice. published by Occupational Diseases and Environmental Medicine, Vol.4 No.2, 2016
  • Conformational Disorder of the Most Immature Cu,Zn-Superoxide Dismutase Leading to Amyotrophic Lateral Sclerosis J. Biol. Chem., pii: jbc.M115.683763.
  • Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture.  Journal of Asian Ceramic Societies ; November 15, 2014
  • Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes.  J Life Sci ; June, 2014
  • A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase.  J Biol Chem ; June 10, 2014
  • Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor. Int J Biochem Cell Biol ; November 11, 2012

[Date : September 24 2020 23:16]

Detail Product Name Product Code Supplier Size Price
Copper Assay kit LS - 3,5-DiBr-PAESA method
DatasheetThis may not be the latest data sheet.
CU03ME AKJMetallogenics Co.,Ltd. 1 kit $365

Description Metallo Assay Copper Assay kit LS is intended for the quantitative determination of copper in biological sample by 96-well reader use. Target: copper (Cu2+ and Cu+). Measuring range: 3-400 μg/Dl Species: all Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 580 nm (570-590nm) Procedure: 10 min
Storage 4°C CAS
Link

Assay Kits Product List
Metallo Assay LS

Copper Assay kit LS - 3,5-DiBr-PAESA method
DatasheetThis may not be the latest data sheet.
CU04ME AKJMetallogenics Co.,Ltd. 1 kit $584

Description Metallo Assay Copper Assay kit LS is intended for the quantitative determination of copper in biological sample by 96-well reader use. Target: copper (Cu2+ and Cu+) Measuring range: 3-400 μg/Dl Species: all Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 580 nm (570-590nm). Procedure: 10 min
Storage 4°C CAS
Link

Assay Kits Product List
Metallo Assay LS

[Date : September 24 2020 23:16]

Copper Assay kit LS - 3,5-DiBr-PAESA method


  • Product Code: CU03ME
  • Supplier: AKJ
  • Size: 1kit
  • Price: $365

Description Metallo Assay Copper Assay kit LS is intended for the quantitative determination of copper in biological sample by 96-well reader use. Target: copper (Cu2+ and Cu+). Measuring range: 3-400 μg/Dl Species: all Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 580 nm (570-590nm) Procedure: 10 min
Storage 4°C CAS
Link

Assay Kits Product List
Metallo Assay LS

Copper Assay kit LS - 3,5-DiBr-PAESA method


  • Product Code: CU04ME
  • Supplier: AKJ
  • Size: 1kit
  • Price: $584

Description Metallo Assay Copper Assay kit LS is intended for the quantitative determination of copper in biological sample by 96-well reader use. Target: copper (Cu2+ and Cu+) Measuring range: 3-400 μg/Dl Species: all Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 580 nm (570-590nm). Procedure: 10 min
Storage 4°C CAS
Link

Assay Kits Product List
Metallo Assay LS

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CONTACT

export@funakoshi.co.jp

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