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HOME> Products> Natural and Chemical compound> TLR9 Agonist D35/K3 (CpG-ODN)

TLR agonist that can effectively activate pDCs B cells TLR9 Agonist D35/K3 (CpG-ODN)

Date：November 15 2016Web Page No：80735

TLR9 Ligand (CpG-ODN) for pDCs and B-cells, D35 and K3
Potent and specific immuno-stimulation for human/mouse immune cells and laboratory animals. Highly pure and endotoxin-free (0.5 EU/mg or less).

What is TLRs
Features
Example Data
References
Product Information

What is TLRs

Toll-like receptors (TLRs) recognize highly conserved structural motifs as one of the early innate immune responses to invading pathogens. TLR9 distinguishes mammalian and bacterial DNA and induces immune cell activation. Synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODNs) stimulate the immune system, promote the maturation and activation of antigen-presenting cells, and promote the induction of Th1 and inflammatory cytokines.
High quality TLR9 agonists to activate immune cells and their respective controls are available.

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Features

Common

Strong and specific immune stimulation for human/mouse immune cells and laboratory animals.
Highly pure and endotoxin-free in sterile vials, ideal for in vivo experiments. (for research use only)
Different classes of TLR9 agonists have been shown to have different effects on cells. You can choose from the following two types of agonists.
Bulk amount and GMP-grade is available.

A Class TLR9 ligand (partial S-DNA)

Induces type I interferon (IFN) production.
Shows low activity against B cells.
Useful for activation of plasmacytoid dendritic cells (pDCs), induction of IFN-α from human peripheral blood mononuclear cells (PBMC), and activation of IFN-signal pathway.

B Class TLR9 ligand (whole S-DNA)

Stimulates B cells and activates TLR9 dependent NF-kB signal.
Shows low activity for IFN-α induction.
Useful for B cell activation, induction of IL-6 from human PBMC, and activation of NF-kB signal pathway.

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Example Data

Cytokine induction in human PBMCs — **Cytokine induction in human PBMC**

Human PBMCs (1×10⁶ cells) were stimulated with 1 μM of each CpG, and concentrations of human IFN-α and IL-6 in the culture supernatant 24 hours after stimulation were measured by ELISA. Bar graphs show mean ±SEM.

Cytokine induction in mouse FL-DCs — **Cytokine induction in mouse FL-DC**

Mouse bone marrow-derived FL-DCs (2×10⁶ cells) induced to differentiate with FLT3L were stimulated with 1 μM of each CpG, and concentrations of mouse IFN-α and IL-6 in the culture supernatant 24 hours after stimulation were measured by ELISA. Bar graph shows mean ±SEM.

* The data was provided as a result of joint research with Dr. Ken Ishii and Dr. Taiki Aoshi at Laboratory of Adjuvant Innovation (at that time), National Institute of Biomedical Innovation.

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References

Ishii, K. J., et al., Curr. Opin. Mol. Ther., 6(2), 166～174(2004).
Verthelyi, D., et al., J. Immunol., 166(4), 2372～2377(2001).

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Product Information

[Date : August 23 2025 00:07]

Detail Product Name Product Code Supplier Size Price

D35 Endotoxin-Free (A-class TLR9 Ligand)
DatasheetThis may not be the latest data sheet. CN-65001 GDI 10 mg $830

Contact

Description	D35 is A-Class TLR9 ligand called CpG-ODN as partial S-DNA and induces high type I IFN production. D35 is useful for activation of plasmacytoid dendric cells (pDCs), induction of IFN-α from PBMCs, and activation of IFN-signal pathways but has low activity against B cells.
Storage	-20°C	CAS
Link

K3 Endotoxin-Free (B-class TLR9 Ligand)
DatasheetThis may not be the latest data sheet. CN-65003 GDI 10 mg $830

Contact

Description	K3 is B-Class TKR9 ligand called CpG-ODN as whole S-DNA and stimulates B cells to activate TLR9-dependent NF- k B signal. K3 is useful for B cell activation, induction of IL -6 from human PBMCs, and activation of the NF-kB signal pathway but has low activity with respect to induction of IFN-α.
Storage	-20°C	CAS
Link

[Date : August 23 2025 00:07]

D35 Endotoxin-Free (A-class TLR9 Ligand)

DatasheetThis may not be the latest data sheet.

Product Code: CN-65001
Supplier: GDI
Size: 10mg
Price: $830

Description	D35 is A-Class TLR9 ligand called CpG-ODN as partial S-DNA and induces high type I IFN production. D35 is useful for activation of plasmacytoid dendric cells (pDCs), induction of IFN-α from PBMCs, and activation of IFN-signal pathways but has low activity against B cells.
Storage	-20°C	CAS
Link

Contact

K3 Endotoxin-Free (B-class TLR9 Ligand)

DatasheetThis may not be the latest data sheet.

Product Code: CN-65003
Supplier: GDI
Size: 10mg
Price: $830

Description	K3 is B-Class TKR9 ligand called CpG-ODN as whole S-DNA and stimulates B cells to activate TLR9-dependent NF- k B signal. K3 is useful for B cell activation, induction of IL -6 from human PBMCs, and activation of the NF-kB signal pathway but has low activity with respect to induction of IFN-α.
Storage	-20°C	CAS
Link

Contact

Control

[Date : August 23 2025 00:07]

Detail Product Name Product Code Supplier Size Price

D35(GC) Endotoxin-Free (A-class TLR9 Ligand Control)
DatasheetThis may not be the latest data sheet. CN-65002 GDI 10 mg $830

Contact

Description
Storage	-20°C	CAS
Link

K3(GC) Endotoxin-Free (B-class TLR9 Ligand Control)
DatasheetThis may not be the latest data sheet. CN-65004 GDI 10 mg $830

Contact

Description
Storage	-20°C	CAS
Link

[Date : August 23 2025 00:07]

D35(GC) Endotoxin-Free (A-class TLR9 Ligand Control)

DatasheetThis may not be the latest data sheet.

Product Code: CN-65002
Supplier: GDI
Size: 10mg
Price: $830

Description
Storage	-20°C	CAS
Link

Contact

K3(GC) Endotoxin-Free (B-class TLR9 Ligand Control)

DatasheetThis may not be the latest data sheet.

Product Code: CN-65004
Supplier: GDI
Size: 10mg
Price: $830

Description
Storage	-20°C	CAS
Link

Contact

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