Keyword	：

Keyword	：
Supplier	：Select	Product code	：
Product type	：	Discontinued product	：

Keyword	：
Supplier	：Select	Product code	：
Discontinued product	：

AntigenHelp	：	Cross reactivityHelp	：	Host animalHelp	：
ConjugationHelp	：	ApplicationHelp	：	PurificationHelp	：
AbsorptionHelp	：	IsotypeHelp	：	CloneHelp	：

Product type	：	Match condition	：	Discontinued product	：

Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：

CAS No	：

Supplier	：Select	Discontinued product	：

WEB page No search	：

HOME> Products> Miscellaneous> Ap3 : Non-fluorescent SHG-Imaging dye
HOME> Products> Cell Culture> Imaging dye> Ap3 : Non-fluorescent SHG-Imaging dye

Membrane potential-sensitive Ap3 : Non-fluorescent SHG-Imaging dye

Date：August 08 2018Web Page No：80646

Funakoshi Co.,Ltd.

Ap3 is the world first Non-fluorescent, photostable SHG-imaging dye. Second harmonic generation (SHG) imaging is a powerful tool to visualize cell / tissue structure and function. However, dyes which have been used so far emit strong fluorescence signals as well as SHG signals, and this fluorescence disturbs multimodal imaging with SHG signal. Ap3 overcomes this disadvantage, and enables true SHG signals in multimodal imaging by no interference of signals from other fluorescent molecules.

Features
Specification
Outline of protocol
Performance data of Ap3
Price of Ap3
Reference of Ap3

Features

SHG-specific dye. No fluorescence signal.
Enables multimodal imaging by using Ap3 and other fluorescent dyes simultaneously.
Photostable
Low phototoxity
Laser illumination : 950 nm / SHG signal detection : 465 - 485 nm

*Two-photon excitation microscopy system and special filter for SHG imaging is required.
*For SHG imaging, the detection system is required to set on the opposition side of the objective lens. Besides, the installation of photomultiplier tube(PMT) is preferable on the detection side.

Go back to index

Specification

Molecular formula : C₃₂H₅₅Br₂N₅
Molecular weight　: 669.6206
Solubility : Water soluble
Purity : >98%
License : This prodcut has been commercialized under a license from Keio University and University of Tsukuba.

Go back to index

Outline of protocol

Cultivate cells at appropriate cell density on glass bottom dishes.
Dilute Ap3 stock solution in an appropriate buffer.
Remove culture medium from glass bottom dishes and add diluted Ap3 (e.g. 200 µL - 1,000 µL), to cells at a final concentration of 20 µM.
Incubate cells for few minutes.
Generate SHG signals with 950 nm laser illumination and observe it with 465 - 485 nm band pass filter through two-photon excitation microscopy system.

Go back to index

Performance data of Ap3

SHG signal and no fluorescence with Ap3 treatment

Membrane potential changes by SHG imaging in brain slices.
a: Membrane potential sensitivity of SHG signals from Ap3 assessed by voltage-clamp.
b: SHG signal changes upon action potential in neurons. SHG signals were monitored by a point-scan protocol at the soma of patch-clamped neurons in brain slices.
Nuriya M., et al, Nat. Commun., 7:11557 (2016).

Simultaneous imaging of membrane potential and intracellular calcium dynamics in cortical neurons.
Nuriya M., et al, Nat. Commun., 7:11557 (2016).

Multimodal two-photon imaging of cell membrane and fluorescence marker.
Blue：Ap3、Green：pHrodo Green、Red：ER-Tracker Red

Go back to index

Price of Ap3

[Date : April 27 2024 00:06]

Detail Product Name Product Code Supplier Size Price

DatasheetThis may not be the latest data sheet. FDV-0008 FNAFunakoshi Co.,Ltd. 1 mg $400

Contact

Description	Ap3 is the world first Non-fluorescent, photostable and membrane potential-sensitive SHG-active organic dye. Second harmonic generation (SHG) imaging is a powerful tool to visualize cell / tissue structure and function, and widely used. However, dyes which have been used so far emit strong fluorescence signals besides SHG signals, and this fluorescence disturbs multimodal imaging with SHG signal. Ap3 overcomes this disadvantage, and enables true SHG signals in multimodal imaging by no interference of signals from other fluorescent molecules.
Storage	-20°C	CAS
Link

[Date : April 27 2024 00:06]

DatasheetThis may not be the latest data sheet.

Product Code: FDV-0008
Supplier: FNA
Size: 1mg
Price: $400

Description	Ap3 is the world first Non-fluorescent, photostable and membrane potential-sensitive SHG-active organic dye. Second harmonic generation (SHG) imaging is a powerful tool to visualize cell / tissue structure and function, and widely used. However, dyes which have been used so far emit strong fluorescence signals besides SHG signals, and this fluorescence disturbs multimodal imaging with SHG signal. Ap3 overcomes this disadvantage, and enables true SHG signals in multimodal imaging by no interference of signals from other fluorescent molecules.
Storage	-20°C	CAS
Link

Contact

Go back to index

Reference of Ap3

Nuriya M., et al, Nat. Commun., 7:11557 (2016).

Go back to index

CONTACT

export@funakoshi.co.jp

※Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
※Please note that Product Information or Price may change without notice.