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HOME> Products> Miscellaneous> Others> Laminin Receptor Specific Peptide : AG73, A208, C16, A3G756

Specifically bind to each laminin receptor Laminin Receptor Specific Peptide : AG73, A208, C16, A3G756

Date：June 20 2016Web Page No：80630

Laminin is a basic component of basement membrane, composed of alpha, beta and gamma subunits. Laminin is also known as a superior cell culture substrate, and its bioactive sequence interacts with integrin, syndecan, etc. It has been thought that specific laminin receptor's function analysis is difficult as various laminin receptors interact with laminin in parallel during cell adhesion.

AG73, A208, C16 and A3G756 are synthetic peptides which have bioactive sequence of laminin. These peptides can be used as a cell culture substrate as they bind to each receptor specifically. Cell function behavior, induced by its specific receptors and downstream signaling can be analyzed by using these peptides.

Features
Example Data
Reference

Features

Peptide (Code)	Description (Sequence)	Receptor	Reported Functions	Reference
AG73 (#KP004)	mouse laminin α1 chain 2719-2730 (RKRLQVQLSIRT)	Syndecan	Strong Cell Adhesion Activity Neurite Elongation Activity Cancer Cell Metastasis to Liver Promotion Activity Cell Differentiation Promotion Activity Vascular Lumen Formation Promotion Activity	1-3
A208 (#KP005)	mouse laminin α1 chain 2097-2108 (AASIKVAVSADR)	IKVAV receptor	Strong Cell Adhesion Activity Neurite Elongation Activity Cancer Cell Metastasis to Liver Promotion Activity Cell Differentiation Promotion Activity Vascular Lumen Formation Promotion Activity Wound Healing Activity	4-6
C16 (#KP006)	mouse laminin γ1 chain 135-150 (KAFDITYVRLKF)	Integrin αvβ1, α5β3	Strong Cell Adhesion Activity Neurite Elongation Activity Cancer Cell Metastasis to Liver Promotion Activity MMP Induction Activity Angiogenesis Promotion Activity	7-9
A3G756 (#KP007)	mouse laminin α3 chain 1411-1429 (KNSFMALYLSKGRLVFALG)	Syndecan	Strong Cell Adhesion Activity Neurite Elongation Activity Wound Healing Activity Adhesion/Migration Activity for Epithelial Cell	10-12

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Example Data

* These data are provided by Professor Motoyoshi Nomizu
(Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science)

Recombinant laminin alpha1 chain (LG4) or AG73 (specific region of LG4) is used as a coating material. Recombinant laminin alpha1 chain (LG4) contains Integrin α2β1 or syndecan binding site, but only AG73 binds to syndecan. Morphology is very different between two coating materials.

Concentration-dependent cell adhesion by each peptide: Each peptide is coated on 96 well culture plate and blocked by BSA. Human Dermal Fibrobrast is cultivated (20,000 cells/well), incubated two hours and fixed by 4% paraformaldehyde. Cells are stained by crystal violet and counted its numbers.

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Reference

Nomizu, et al., J Biol. Chem., 270, 20583 - 20590 (1995).
Hoffman, et al., J Biol. Chem., 273, 28633 - 28641 (1998).
Mochizuki, et al., Arch. Biochem. Biophys., 459, 249 - 255 (2007).
Nomizu, et al., J Biol. Chem., 273, 32491 - 32499 (1998).
Kasai, et al., Biopolymers(Peptide Science), 76, 27 - 33 (2004).
Kasai, et al., Biochemistry, 46, 3966 - 3974 (2007).
Nomizu, et al., J Biol. Chem., 272, 32198 - 32205 (1997).
Ponce, et al., Circ Res., 84, 688 - 694 (1999).
Christos, et al., Experimental Cell Research, 260, 268 - 276 (2000).
Utani, et al., J Biol. Chem., 276, 28779 - 28788 (2001).
Kato, et al., Biochemistry, 41, 10747 - 10753 (2002).
Utani, et al., J Biol. Chem., 278, 34483 - 34490 (2003).

[Date : February 15 2024 12:37]

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