HOME> Products> Cell Culture> "Prote-in" Transfection Reagent
HOME> Products> Protein / Enzyme> "Prote-in" Transfection Reagent
HOME> Products> Export Business> Protein > Enzyme> Protein Transfection Reagent> "Prote-in" Transfection Reagent

Peptide-base Protein Transfection Reagent for Cells "Prote-in" Transfection Reagent

Date:March 09 2016Web Page No:80517

"Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.

Peptide transporter "Prote-in" consists of 3 region of peptide sequences - Hydrophobic region, Linker and Cell Penetrating Peptide (CPP) area. Hydrophobic region binds to Proteins. CPP region helps to corporate the protein-transporter complex into cytoplasm by binding to heparan sulfate proteoglycan on the cell surface. As transporter and protein do not bind covalently, protein function is maintained.

Features of "Prote-in"

  • Low cytotoxicity
  • Rapid - Complex is structured in a hour, and transfection is completed in 3 hours.
  • Versatility - Can be used for both Adherent and Non-adherent Cells.

Go back to index

Principle of "Prote-in"

Principle of Prote-in

Go back to index

Protocol Outline

Protocol Outline of Prote-in

Go back to index

Example of Use

HeLa-S3 Cell Line (Human Uterus Cancer : Adherent Cell)

0.1 uM FITC labeled Antibody(150kDa)
+ 0.25 uM "Prote-in" Reagent 0.1 uM FITC labeled Antibody(150kDa) Prote-in was used Prote-in wasn't used

FITC labeled Antibody : Anti-Sheep IgG (Whole Molecule), FITC Conjugate, Donkey Polyclonal (SIGMA)

Go back to index

Examples of Cells Transfected Successfully

Adherent Cells Non - Adherent Cells HeLa-S3 cell line K562 cell line WiDr cell line Jurkat cell line HepG2 cell line HL60 cell line MCF-7 cell line P388 cell Balb3T3 cell line P3U1 cell B16 cell line SVT2 cell line A10 cell line

Go back to index

Comparison Data

FITC labeled Antibody (150kDa) Transfection to HeLa-S3 Cell Line

Prote-in
0.25 uM "Prote-in" Reagent Company B
Following by recommended protocol Company C
Following by recommended protocol Prote-in was used Comparison Data to Prote-in Comparison Data to Prote-in

β-galactosidase (465kDa) Transfection to HeLa-S3 Cell Line

Prote-in
0.25uM "Prote-in" Reagent Company B
Following by recommended protocol Company C
Following by recommended protocol Prote-in was used Comparison Data to Prote-in Comparison Data to Prote-in

Go back to index

Price of Prote-in

[Date : August 22 2019 23:13]

Detail Product Name Product Code Supplier Size Price
”Prote-in” Transfection Reagent
160-0526-0 HYG 6 tests $200

Description "Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.
Storage -80°C CAS
Link

”Prote-in” Transfection Reagent
160-0518-0 HYG 24 tests $600

Description "Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.
Storage -80°C CAS
Link

[Date : August 22 2019 23:13]

”Prote-in” Transfection Reagent


  • Product Code: 160-0526-0
  • Supplier: HYG
  • Size: 6tests
  • Price: $200

Description "Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.
Storage -80°C CAS
Link

”Prote-in” Transfection Reagent


  • Product Code: 160-0518-0
  • Supplier: HYG
  • Size: 24tests
  • Price: $600

Description "Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.
Storage -80°C CAS
Link

Go back to index

CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.